Details

Autor(en) / Beteiligte

• Liew, Jeremy J.M.
• Wicht, Denyce K.
• Gonzalez, Reyaz
• Dowling, Daniel P.
• Ellis, Holly R.

Titel

Current understanding of enzyme structure and function in bacterial two-component flavin-dependent desulfonases: Cleaving C–S bonds of organosulfur compounds

Ist Teil von

• Archives of biochemistry and biophysics, 2024-08, Vol.758, p.110048, Article 110048

Ort / Verlag

Elsevier Inc

Erscheinungsjahr

2024

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The inherent structural properties of enzymes are critical in defining catalytic function. Often, studies to evaluate the relationship between structure and function are limited to only one defined structural element. The two-component flavin-dependent desulfonase family of enzymes involved in bacterial sulfur acquisition utilize a comprehensive range of structural features to carry out the desulfonation of organosulfur compounds. These metabolically essential two-component FMN-dependent desulfonase systems have been proposed to utilize oligomeric changes, protein-protein interactions for flavin transfer, and common mechanistic steps for carbon-sulfur bond cleavage. This review is focused on our current functional and structural understanding of two-component FMN-dependent desulfonase systems from multiple bacterial sources. Mechanistic and structural comparisons from recent independent studies provide fresh insights into the overall functional properties of these systems and note areas in need of further investigation. The review acknowledges current studies focused on evaluating the structural properties of these enzymes in relationship to their distinct catalytic function. The role of these enzymes in maintaining adequate sulfur levels, coupled with the conserved nature of these enzymes in diverse bacteria, underscore the importance in understanding the functional and structural nuances of these systems. [Display omitted] •Gene organization of enzymes involved in sulfur acquisition.•Structure and function of the two-component FMN-dependent desulfonase systems.•Proposed mechanism of desulfonation by the desulfonase enzymes.•Protein-protein interactions involved in flavin transfer.