Details

Autor(en) / Beteiligte

• Li, Cun-Yu
• Hu, Si-Yi
• Yang, Wen-Ting
• Yang, Hong-Zhiyuan
• Zhang, Wen-Wen
• Ye, Jian-Hui
• Zheng, Xin-Qiang
• Liang, Yue-Rong
• Dong, Zhan-Bo
• Lu, Jian-Liang

Titel

Conversion obstacle from Mg-protoporphyrin IX to protochlorophyllide might be responsible for chlorophyll-deficient phenotype of the Huangjinya's albino offspring

Ist Teil von

• Plant physiology and biochemistry, 2024-07, Vol.212, p.108778, Article 108778

Ort / Verlag

France: Elsevier Masson SAS

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• The albino tea cultivar is one of the most important germplasms for key gene mining and high-quality tea producing. In order to elucidate the chlorophyll-deficient mechanism of albino cultivar 'Huangjinya' and its offspring, color difference, photosynthetic pigments and the relevant genes’ expression of the tender shoots were comprehensively investigated in this study. Among the tested 16 offspring, 5 exhibited albino phenotype in spring and autumn, 3 showed albino phenotype in spring but normal green in autumn, while the rests were all normal green. The shoot of albino offspring had significantly higher lightness and/or yellowness than that of green ones, and possessed dramatically lower photosynthetic pigments and chlorophyll precursor protochlorophyllide (Pchlide), as well as higher chlorophyll a/chlorophyll b but lower chlorophylls/carotenoids in comparison with green ones. Among the tested genes involved in chlorophyll and carotenoid metabolism pathways, expression of the magnesium protoporphyrin IX monomethyl ester cyclase (CRD), 3,8-divinyl chlorophyllide 8-vinyl reductase (DVR), 5-aminolevulinate dehydratase 1 (HEMB1), 1-deoxy-D-xylulose 5-phosphate synthase 1 (DXS1) and 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ISPH) was remarkably down-regulated in shoots of the albino offspring. Color difference indices of the offspring were significantly correlated with the levels of photosynthetic pigments and Pchlide, and low level of chlorophylls in shoot of albino offspring was mainly due to conversion obstacle from magnesium protoporphyrin Ⅸ (Mg-Proto IX) to Pchlide which might be attributed to down-regulatory expression of CRD and DVR. [Display omitted] •Shoots of albino germplasm possessed lower chlorophylls and the precursor Pchlide.•Expression of CRD, DVR, HEMB1, DXS1 and ISPH was down-regulated in these shoots.•Conversion from Mg-protoporphyrin Ⅸ to Pchlide was inhibited in albino germplasm.•Conversion obstacle might be due to down-regulatory expression of CRD and DVR.