Details

Autor(en) / Beteiligte

• Li, Ming
• Teng, Weipeng
• Lu, Wenying
• Sun, Mingna
• Duan, Jinsheng
• Qiu, Xuchun

Titel

Exo I-based cyclic digestion coupled with synergistic enhancement strategy for integrating dual-mode optical aptasensor platform

Ist Teil von

• Talanta (Oxford), 2024-08, Vol.276, p.126286, Article 126286

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2024

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The improvement of dual-mode techniques was of particular interest to researchers, which might enhance the detection performance and applicability. Here, a dual-mode optical aptasensor (DO-aptasensor) platform based on exonuclease I (Exo I) cyclic digestion and synergistic enhancement strategy had proposed for zearalenone (ZEN). Following the preparation of dumbbell-shaped signal probe, the Exo I-based cyclic digestion amplification performed, and then the synergistic enhancement effect carried out to achieve the Poly-HRP-based colorimetry and FAM-SGI-based fluorescence. The efficient homogeneous system realized through the magnetic separation, while the signal interference further eliminated by the graphene oxide (GO). The LOD values were as low as 0.067 ng mL−1 for colorimetry mode and 0.009 ng mL−1 for fluorescence mode, which reduced 23-fold and 172-fold than ELASA by same ZEN-Apt. This promising platform gave rise to a dual-mode optical readout, improved sensitivity and positively correlated detection. Meanwhile, the DO-aptasensor also exhibited the acceptable specificity, desirable reliability and excellent practicability. This novel avenue of aptasensor platform hold great potential for dual-mode optical monitoring of other targets, which can further expand the application scope of Exo I-based signal amplification and synergistic enhancement effect. [Display omitted] •Dual-mode aptasensor: Poly-HRP-based colorimetry and FAM-SGI-based fluorescence.•Efficient homogeneous system through MB, and minimized signal interference by GO.•Positively correlated LOD were as low as 0.067 ng mL−1 and 0.009 ng mL−1.•Sensitivity, reliability and practicability had been well exhibited.•Application of cyclic digestion and synergistic enhancement could broaden.