Details

Autor(en) / Beteiligte

• Wu, Zhigang
• Guo, Shuo
• Yue, Haixia
• Huang, Hai
• Zhang, Ziru
• Chen, Xuexue
• Lan, Chaoyu
• Ren, Lu
• Hei, Peng
• Wang, Xinyu
• Wang, Ning
• Li, Xiaokang

Titel

Effect of M2-macrophage treated lymphatic endothelial cells on angiogenesis that promoted osteointegration

Ist Teil von

• Experimental cell research, 2024-06, Vol.439 (1), p.114096-114096, Article 114096

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2024

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Early vascularization plays an essential role during the whole process in bone regeneration because of the function of secreting cytokines, transporting nutrients and metabolic wastes. As the preliminary basis of bone repair, angiogenesis is regulated by immune cells represented by macrophages to a great extent. However, with the discovery of the endolymphatic circulation system inside bone tissue, the role of vascularization became complicated and confusing. Herein, we developed a macrophage/lymphatic endothelial cells (LECs)/human umbilical vein endothelial cells (HUVECs) co-culture system to evaluate the effect of macrophage treated lymphatic endothelial cells on angiogenesis in vitro and in vivo. In this study, we collected the medium from macrophage (CM) for LECs culture. We found that CM2 could promote the expression of LECs markers and migration ability, which indicated the enhanced lymphogenesis. In addition, the medium from LECs was collected for culturing HUVECs. The CM2-treated LECs showed superior angiogenesis property including the migration capacity and expression of angiogenetic markers, which suggested the superior vascularization. Rat femoral condyle defect model was applied to confirm the hypothesis in vivo. Generally, M2-macrophage treated LECs showed prominent angiogenetic potential coupling with osteogenesis. •M2 macrophage promotes the lymphogenesis in vitro and in vivo.•M2 macrophage promotes the upregulation of CXCL-12 in LECs.•M2 macrophage promotes LECs-induced angiogenetic differentiation.•M2 macrophage promotes LECs-induced osteointegration.