Details

Autor(en) / Beteiligte

• Zagui, Guilherme Sgobbi
• de Almeida, Otávio Guilherme Gonçalves
• Moreira, Natália Columbaro
• Silva, Nicolas Gabriel Aziani
• Meschede, Marina Smidt Celere
• Darini, Ana Lúcia Costa
• Andrade, Leonardo Neves
• Segura-Muñoz, Susana Inés

Titel

Hospital wastewater as source of human pathogenic bacteria: A phenotypic and genomic analysis of international high-risk clone VIM-2-producing Pseudomonas aeruginosa ST235/O11

Ist Teil von

• Environmental research, 2024-08, Vol.255, p.119166, Article 119166

Ort / Verlag

Netherlands: Elsevier Inc

Erscheinungsjahr

2024

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Pseudomonas aeruginosa belong to the special pathogen group capable of causing serious infections, with high mortality rates. The aim of this study was to describe the antibiotic resistance and genomic characteristics of Pseudomonas aeruginosa belonging to international high-risk clone ST235 (GPAE0131 isolate), obtained from hospital wastewater. P. aeruginosa GPAE0131 was isolated from ward tertiary hospital in Brazil and the antibiotic resistance profile was determined by the disc-diffusion method. Genomic characteristics related to antibiotic resistance and virulence factors were evaluated by genomic DNA sequencing on the Illumina MiSeq platform and bioinformatic analysis. GPAE0131 isolate showed resistance to piperacillin-tazobactam, cefepime, ceftazidime, imipenem, meropenem, ciprofloxacin, levofloxacin and tobramycin. Resistome comprehend of resistance genes to β-lactams (blaVIM-2, blaOXA-4, blaOXA-488, blaPDC-35), aminoglycosides (aph(3′)-IIb, aac(6′)-IIc, aac(6′)-Ib9, aadA1), fosfomycin (fosA), chloramphenicol (catB7) and sulfonamides (sul1). Genome comparisons evidence insertion of blaVIM-2 and blaOXA-4 genes. GPAE0131 isolate was predicted to be pathogenic to humans and several virulence factors were found, including encoding gene for ExoU and exotoxin A. All of these features into a pathogenic international high-risk clone (ST235), classified as critical priority, stands out as public health concern due to the widespread dispersal of human pathogens through wastewater. It is suggested that mitigating measures be implemented, such as the treatment of hospital sewage and the addition of tertiary treatment, to prevent the escape of pathogens at this level into the environment. •Carbapenem-resistant Pseudomonas aeruginosa was isolated from hospital wastewater.•The isolate belongs to international high-risk clone (ST235) and O11 serotype.•VIM-2 metallo-β-lactamase encoding gene was detected (blaVIM-2).•Genomic comparisons shown genetic insertions of blaVIM-2 and blaOXA-4.•Clinically relevant virulence factors were detected (exoU and toxA).