Details

Autor(en) / Beteiligte

• Lima, Eduarda S.
• de Araújo Pinheiro, Aglaete
• da Rocha, Cláudia Quintino
• e Silva, Carolina Rocha
• Costa-Junior, Livio Martins
• Alencar, Luciana Magalhães Rebêlo
• Soares, Alexandra Martins Santos

Titel

An alkaloidal fraction of Annona tomentosa possesses anthelmintic activity and induces surface modifications in Haemonchus contortus

Ist Teil von

• Veterinary parasitology, 2024-07, Vol.329, p.110195, Article 110195

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2024

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The nematode Haemonchus contortus is, as a parasite, responsible for most mortality of small ruminants, causing significant economic losses. Numerous plant-derived compounds have exhibited promising anthelmintic activities against this nematode. Notably, the Annona genus stands out for demonstrated anthelmintic effects by extracts from several of its species against different nematodes. This study aimed to assess the effect of an Annona tomentosa fraction, rich in alkaloids, on H. contortus. This fraction, named Alk.F, is derived from the methanolic extract of the plant’s stem bark. Chemical characterization of Alk.F was performed by liquid chromatography coupled with mass spectrometry. Among the nine predominant peaks obtained, seven alkaloids were identified: reticuline, reticuline N-oxide, reticuline N-oxide isomer, cyclanoline, asimilobine, tetrahydropalmatine and anonaine. Alk.F inhibited the larval development of H. contortus with an IC50 of 0.026 mg/mL, inhibited larval exsheathment with an IC50 of 0.38 mg/mL, and displayed low hemolytic activity towards sheep erythrocytes. Furthermore, atomic force microscopy revealed that Alk.F altered adhesive forces and the height profile on the surface of H. contortus larvae. In conclusion, A. tomentosa alkaloids alter the cuticle structure of H. contortus, inhibiting larval development and exsheathment, thus offering possibilities for contributing to the development of new anthelmintic drugs. •An Annona tomentosa alkaloidal fraction is in vitro effective towards Haemonchus contortus.•The A. tomentosa alkaloids exhibit a low in vitro hemolytic activity.•Atomic Force Microscopy reveals that the alkaloids modify the nematode cuticle.