Details

Autor(en) / Beteiligte

• Li, Xiao-Hua
• Duan, Jian-Lu
• Ma, Jing-Ya
• Liu, Xiao-Yu
• Sun, Xiao-Dong
• Wang, Yue
• Tan, Miao-Miao
• Yuan, Xian-Zheng

Titel

Probing the Surface Layer Modulation on Archaeal Mechanics and Adhesion at the Single-Cell Level

Ist Teil von

• Analytical chemistry (Washington), 2024-06, Vol.96 (22), p.8981-8989

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• Addressing the challenge of understanding how cellular interfaces dictate the mechanical resilience and adhesion of archaeal cells, this study demonstrates the role of the surface layer (S-layer) in methanogenic archaea. Using a combination of atomic force microscopy and single-cell force spectroscopy, we quantified the impact of S-layer disruption on cell morphology, mechanical properties, and adhesion capabilities. We demonstrate that the S-layer is crucial for maintaining cell morphology, where its removal induces significant cellular enlargement and deformation. Mechanical stability of the cell surface is substantially compromised upon S-layer disruption, as evidenced by decreased Young’s modulus values. Adhesion experiments revealed that the S-layer primarily facilitates hydrophobic interactions, which are significantly reduced after its removal, affecting both cell–cell and cell–bubble interactions. Our findings illuminate the S-layer’s fundamental role in methanogen architecture and provide a chemical understanding of archaeal cell surfaces, with implications for enhancing methane production in biotechnological applications.