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OsLSC6 Regulates Leaf Sheath Color and Cold Tolerance in Rice Revealed by Metabolite Genome Wide Association Study
Ist Teil von
Rice (New York, N.Y.), 2024-12, Vol.17 (1), p.34-34
Ort / Verlag
New York: Springer US
Erscheinungsjahr
2024
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Plant metabolites including anthocyanins play an important role in the growth of plants, as well as in regulating biotic and abiotic stress responses to the environment. Here we report comprehensive profiling of 3315 metabolites and a further metabolic-based genome-wide association study (mGWAS) based on 292,485 SNPs obtained from 311 rice accessions, including 160 wild and 151 cultivars. We identified hundreds of common variants affecting a large number of secondary metabolites with large effects at high throughput. Finally, we identified a novel gene namely
OsLSC6
(
Oryza sativa
leaf sheath color 6), which encoded a UDP 3-
O
-glucosyltransferase and involved in the anthocyanin biosynthesis of Cyanidin-3-Galc (sd1825) responsible for leaf sheath color, and resulted in significant different accumulation of sd1825 between wild (purple) and cultivars (green). The results of knockout transgenic experiments showed that
OsLSC6
regulated the biosynthesis and accumulation of sd1825, controlled the purple leaf sheath. Our further research revealed that
OsLSC6
also confers resistance to cold stress during the seedling stage in rice. And we identified that a SNP in
OsLSC6
was responsible for the leaf sheath color and chilling tolerance, supporting the importance of
OsLSC6
in plant adaption. Our study could not only demonstrate that
OsLSC6
is a vital regulator during anthocyanin biosynthesis and abiotic stress responses, but also provide a powerful complementary tool based on metabolites-to-genes analysis by mGWAS for functional gene identification andpromising candidate in future rice breeding and improvement.