Details

Autor(en) / Beteiligte

• Lodovici, Arianna
• Buoso, Sara
• Miras-Moreno, Begoña
• Lucini, Luigi
• Garcia-Perez, Pascual
• Tomasi, Nicola
• Pinton, Roberto
• Zanin, Laura

Titel

Peculiarity of the early metabolomic response in tomato after urea, ammonium or nitrate supply

Ist Teil von

• Plant physiology and biochemistry, 2024-06, Vol.211, p.108666-108666, Article 108666

Ort / Verlag

France: Elsevier Masson SAS

Erscheinungsjahr

2024

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Nitrogen (N) is the nutrient most applied in agriculture as fertilizer (as nitrate, Nit; ammonium, A; and/or urea, U, forms) and its availability strongly constrains the crop growth and yield. To investigate the early response (24 h) of N-deficient tomato plants to these three N forms, a physiological and molecular study was performed. In comparison to N-deficient plants, significant changes in the transcriptional, metabolomic and ionomic profiles were observed. As a probable consequence of N mobility in plants, a wide metabolic modulation occurred in old leaves rather than in young leaves. The metabolic profile of U and A-treated plants was more similar than Nit-treated plant profile, which in turn presented the lowest metabolic modulation with respect to N-deficient condition. Urea and A forms induced some changes at the biosynthesis of secondary metabolites, amino acids and phytohormones. Interestingly, a specific up-regulation by U and down-regulation by A of carbon synthesis occurred in roots. Along with the gene expression, data suggest that the specific N form influences the activation of metabolic pathways for its assimilation (cytosolic GS/AS and/or plastidial GS/GOGAT cycle). Urea induced an up-concentration of Cu and Mn in leaves and Zn in whole plant. This study highlights a metabolic reprogramming depending on the N form applied, and it also provide evidence of a direct relationship between urea nutrition and Zn concentration. The understanding of the metabolic pathways activated by the different N forms represents a milestone in improving the efficiency of urea fertilization in crops. [Display omitted] •Ammonium induce an up concentration of Gln and a down concentration of Glu in leaves.•Asn is accumulated in leaves and roots irrespective from the nitrogen-form applied.•Plant secondary metabolism is particularly modulated by ammonium and urea.•Zinc concentration have a direct relationship with the nitrogen source supplied.