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Details

Autor(en) / Beteiligte
Titel
Detection of mpox virus in wastewater provides forewarning of clinical cases in Canadian cities
Ist Teil von
  • The Science of the total environment, 2024-07, Vol.933, p.173108-173108, Article 173108
Ort / Verlag
Netherlands: Elsevier B.V
Erscheinungsjahr
2024
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
  • Wastewater-based surveillance (WBS) has shown to be an effective tool in monitoring the spread of SARS-CoV-2 and has helped guide public health actions. Consequently, WBS has expanded to now include the monitoring of mpox virus (MPXV) to contribute to its mitigation efforts. In this study, we demonstrate a unique sample processing and a molecular diagnostic strategy for MPXV detection that can inform on the epidemiological situation of mpox outbreaks through WBS. We conducted WBS for MPXV in 22 Canadian wastewater treatment plants (WWTPs) for 14 weeks. Three MPXV qPCR assays were assessed in this study for the detection of MPXV which include the G2R assays (G2R_WA and G2R_G) developed by the Centers for Disease Control and Prevention (CDC) in 2010, and an in-house-developed assay that we have termed G2R_NML. The G2R_NML assay was designed using reference genomes from the 2022 MPXV outbreak and provides a larger qPCR amplicon size to facilitate Sanger sequencing. Results show that all three assays have similar limits of detection and are able to detect the presence of MPXV in wastewater. The G2R_NML assay produced a significantly greater number of Sanger sequence-confirmed MPXV results compared to the CDC G2R assays. Detection of MPXV was possible where provincial surveillance indicated overall low caseloads, and in some sites forewarning of up to several weeks was observed. Overall, this study proposes that WBS of MPXV provides additional information to help fill knowledge gaps in clinical case-surveillance and is potentially an essential component to the management of mpox. Workflow to detect MPXV in wastewater. Wastewater-based surveillance (WBS) can be used to monitor the spread of MPXV in a broad, equitable, and anonymous manner. Symptomatic and pre-symptomatic individuals shed the virus which ultimately ends up in wastewater. From there, samples can be obtained from wastewater treatment plants, sent to laboratories, and have the nucleic acids extracted to perform real-time qPCR analysis. The generated data is then communicated to the site which helps provide forewarning on the initial emergence of mpox as well as monitor its prevalence over time. (Image created in part using the University of Maryland Center for Environmental Sciences Integration and Application Network: https://ian.umces.edu/symbols/#_Download). [Display omitted] •Wastewater-based surveillance of mpox virus can provide clinical case forewarning.•The performance of three mpox virus qPCR assays was compared.•A new qPCR assay was developed that allows for Sanger sequencing of amplicons.•Despite the low viral load, mpox virus can be successfully monitored in wastewater.
Sprache
Englisch
Identifikatoren
ISSN: 0048-9697
eISSN: 1879-1026
DOI: 10.1016/j.scitotenv.2024.173108
Titel-ID: cdi_proquest_miscellaneous_3053974574

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