Details

Autor(en) / Beteiligte

• Yao, Feifan
• Zhou, Suiqing
• Zhang, Ruizhi
• Chen, Yining
• Huang, Wei
• Yu, Kai
• Yang, Nanmu
• Qian, Xiangjun
• Tie, Xiaofeng
• Xu, Jiali
• Zhang, Yu
• Baheti, Tasiken
• Xu, Jing
• Dai, Xinzheng
• Hao, Xiaopei
• Zhang, Liren
• Wang, Xuehao
• Li, Qing

Titel

CRISPR/Cas9 screen reveals that targeting TRIM34 enhances ferroptosis sensitivity and augments immunotherapy efficacy in hepatocellular carcinoma

Ist Teil von

• Cancer letters, 2024-07, Vol.593, p.216935, Article 216935

Ort / Verlag

Ireland: Elsevier B.V

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• Hepatocellular carcinoma (HCC) is a prevalent malignancy characterized by complex heterogeneity and drug resistance. Resistance to ferroptosis is closely related to the progression of HCC. While HCC tumors vary in their sensitivity to ferroptosis, the precise factors underlying this heterogeneity remain unclear. In this study, we sought to elucidate the mechanisms that contribute to ferroptosis resistance in HCC. Whole-genome CRISPR/Cas9 screen using a subtoxic concentration (IC20) of ferroptosis inducer erastin in the HCC cell line Huh7 revealed TRIM34 as a critical driver of ferroptosis resistance in HCC. Further investigation revealed that TRIM34 suppresses ferroptosis in HCC cells, promoting their proliferation, migration, and invasion both in vitro and in vivo. Furthermore, TRIM34 expression is elevated in HCC tumor tissues, correlating with a poor prognosis. Mechanistically, TRIM34 directly interacts with Up-frameshift 1 (UPF1), a core component of the nonsense-mediated mRNA decay (NMD) pathway, to promote its ubiquitination and degradation. This interaction suppresses GPX4 transcript degradation, thus promoting the protein levels of this critical ferroptosis suppressor in HCC. In light of the close crosstalk between ferroptosis and the adaptive immune response in cancer, HCC cells with targeting knockdown of TRIM34 exhibited an improved response to anti-PD-1 treatment. Taken together, the TRIM34/UPF1/GPX4 axis mediates ferroptosis resistance in HCC, thereby promoting malignant phenotypes. Targeting TRIM34 may thus represent a promising new strategy for HCC treatment. [Display omitted] •Genome-wide CRISPR/Cas9 screen identifies TRIM34 as an important regulator of ferroptosis in HCC cells.•Elevated expression of TRIM34 is associated with poor survival of patients with HCC.•YY1 transcriptionally activates TRIM34, which induces ubiquitin-mediated degradation of UPF1, thus promoting GPX4 expression.•Targeting TRIM34 enhances the efficacy of immunotherapy in HCC.