Details

Autor(en) / Beteiligte

• Shen, Danning
• Zhu, Yingjie
• Mao, Jie
• Lin, Runfeng
• Jiang, Xin
• Liang, Longhui
• Peng, Jing
• Cao, Yanqing
• Dong, Suhe
• He, Kun
• Wang, Na

Titel

Highly sensitive and accurate measurement of underivatized phosphoenolpyruvate in plasma and serum via EDTA-facilitated hydrophilic interaction liquid chromatography–tandem mass spectrometry

Ist Teil von

• Talanta (Oxford), 2024-08, Vol.275, p.126134-126134, Article 126134

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• Phosphoenolpyruvate (PEP) is an essential intermediate metabolite that is involved in various vital biochemical reactions. However, achieving the direct and accurate quantification of PEP in plasma or serum poses a significant challenge owing to its strong polarity and metal affinity. In this study, a sensitive method for the direct determination of PEP in plasma and serum based on ethylenediaminetetraacetic acid (EDTA)-facilitated hydrophilic interaction liquid chromatography–tandem mass spectrometry was developed. Superior chromatographic retention and peak shapes were achieved using a zwitterionic stationary-phase HILIC column with a metal-inert inner surface. Efficient dechelation of PEP–metal complexes in serum/plasma samples was achieved through the introduction of EDTA, resulting in a significant enhancement of the PEP signal. A PEP isotopically labelled standard was employed as a surrogate analyte for the determination of endogenous PEP, and validation assessments proved the sensitivity, selectivity, and reproducibility of this method. The method was applied to the comparative quantification of PEP in plasma and serum samples from mice and rats, as well as in HepG2 cells, HEK293T cells, and erythrocytes; the results confirmed its applicability in PEP-related biomedical research. The developed method can quantify PEP in diverse biological matrices, providing a feasible opportunity to investigate the role of PEP in relevant biomedical research. [Display omitted] •A novel, facile and accurate method for the direct determination of PEP in complex biological matrices is developed.•EDTA is an indispensable additive to plasma/serum to dechelate PEP-metal adducts and release free PEP molecules.•The developed method provides a feasible way for delving deeper the significance of PEP in relevant biomedical research.