Details

Autor(en) / Beteiligte

• Zhang, Fan
• Liu, Fangjia
• Sheng, Xijing
• Liu, Quan
• Cui, Luqing
• Cao, Zhengzheng
• Hu, Tianyu
• Li, Donghua
• Dai, Menghong

Titel

Bacitracin-resistant Staphylococcus aureus induced in chicken gut and in vitro under bacitracin exposure

Ist Teil von

• Microbial pathogenesis, 2024-06, Vol.191, p.106666-106666, Article 106666

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2024

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• It is common knowledge that prolonged and excessive use of antibiotics can lead to antimicrobial resistance. However, the characteristics and mechanism of resistant-bacteria induced by clinically recommended and prophylactic dose drugs remain largely unclear. This study aimed to observe the trends of drug resistance of the bacitracin-susceptible Staphylococcus aureus strain FS127 under exposure to bacitracin (BAC), which were induced in vitro and in chicken gut. Antimicrobial susceptibility testing was used to detect the susceptibility of S. aureus induced in vitro and in the chicken gut to gentamicin, chloramphenicol, tetracycline, doxycycline, penicillin and chloramphenicol. The research results showed that bacitracin could induce drug resistance in S. aureus both in vitro and in vivo. The bacitracin-resistance rate of S. aureus isolated from chicken gut was positively correlated with the dose and time of bacitracin administration. The findings revealed that bacitracin-resistant S. aureus induced in vivo had enhanced susceptibility to chloramphenicol but no such change in vitro. Meanwhile, RT-qPCR assay was used to detect the expression levels of vraD, braD, braR and bacA in typical strains with different bacitracin-resistance levels. It was found that BacA may play a key role in the bacitracin resistance of S. aureus. In conclusion, this work reveals the characteristics and mechanism of bacitracin-resistant S. aureus induced by bacitracin in vivo and in vitro respectively. •S. aureus can be easily induced to a high level (32 μg/mL) of bacitracin resistance under exposure to bacitracin.•The treatment of bacitracin to infected chicken can lead to an increase in bacitracin-resistant S. aureus.•Bacitracin-resistant S. aureus strains induced in vitro show collateral sensitivity to chloramphenicol.•The expression of bacA may play an important role in bacitracin-resistant S. aureus induced by bacitracin.