Journal of cellular and molecular medicine, 2024-04, Vol.28 (8), p.e18356-n/a
2024
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- Autor(en) / Beteiligte
- Titel
- Anti‐inflammatory effect of Trichospira verticillata via suppression of the NLRP3 inflammasome in neutrophilic asthma
- Ist Teil von
- Journal of cellular and molecular medicine, 2024-04, Vol.28 (8), p.e18356-n/a
- Ort / Verlag
- England: John Wiley & Sons, Inc
- Erscheinungsjahr
- 2024
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Trichospira verticillata is an annual herb that belongs to the family Asteraceae. Trichospira verticillata extract (TVE) elicits anti‐plasmodial activity; however, there has been no detailed report about its anti‐inflammatory effects and molecular mechanisms. In addition, herbal plants exhibit anti‐inflammatory effects by suppressing the NLRP3 inflammasome. Therefore, the primary goal of this study was to examine the effects of TVE on NLRP3 inflammasome activation by measuring interleukin‐1β (IL‐1β) secretion. We treated lipopolysaccharides (LPS)‐primed J774A.1 and THP‐1 cells with TVE, which attenuated NLRP3 inflammasome activation. Notably, TVE did not affect nuclear factor‐kappa B (NF‐κB) signalling or intracellular reactive oxygen species (ROS) production and potassium efflux, suggesting that it inactivates the NLRP3 inflammasome via other mechanisms. Moreover, TVE suppressed the formation of apoptosis‐associated speck‐like protein (ASC) speck and oligomerization. Immunoprecipitation data revealed that TVE reduced the binding of NLRP3 to NIMA‐related kinase 7 (NEK7), resulting in reduced ASC oligomerization and speck formation. Moreover, TVE alleviated neutrophilic asthma (NA) symptoms in mice. This study demonstrates that TVE modulates the binding of NLPR3 to NEK7, thereby reporting novel insights into the mechanism by which TVE inhibits NLRP3 inflammasome. These findings suggest TVE as a potential therapeutic of NLRP3 inflammasome‐mediated diseases, particularly NA.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1582-1838eISSN: 1582-4934DOI: 10.1111/jcmm.18356Titel-ID: cdi_proquest_miscellaneous_3047943194
- Format
- –
- Schlagworte
- Alzheimer's disease, Animals, Anti-Inflammatory Agents - pharmacology, Antibodies, Apoptosis, Asthma, Asthma - drug therapy, Asthma - immunology, Asthma - metabolism, Asthma - pathology, Cell culture, Disease Models, Animal, Humans, Immunoprecipitation, Inflammasomes, Inflammasomes - metabolism, Inflammation, Interleukin-1beta - metabolism, interleukin‐1β, Intracellular signalling, Kinases, Leukocytes (neutrophilic), Lipopolysaccharides, Mice, Molecular modelling, neutrophilic asthma, Neutrophils - drug effects, Neutrophils - immunology, Neutrophils - metabolism, NF-kappa B - metabolism, NF-κB protein, NIMA-Related Kinases - metabolism, NLR Family, Pyrin Domain-Containing 3 Protein - metabolism, NLRP3 inflammasome, Oligomerization, Pathogenesis, Phosphatase, Plant Extracts - pharmacology, Proteins, Reactive oxygen species, Reactive Oxygen Species - metabolism, Signal Transduction - drug effects, THP-1 Cells, Trichospira verticillata