Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Ergebnis 9 von 134

Details

Autor(en) / Beteiligte
Titel
Validation of a severe acute respiratory syndrome coronavirus 2 microneutralization assay for evaluation of vaccine immunogenicity
Ist Teil von
  • Journal of virological methods, 2024-06, Vol.327, p.114945-114945, Article 114945
Ort / Verlag
Netherlands: Elsevier B.V
Erscheinungsjahr
2024
Quelle
MEDLINE
Beschreibungen/Notizen
  • As variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continue to emerge, assessment of vaccine immunogenicity remains a critical factor to support continued vaccination. To this end, an in vitro microneutralization (MN50) assay was validated to quantitate SARS-CoV-2 neutralizing antibodies against prototype and variant strains (Beta, Delta, Omicron BA.1, Omicron BA.5, and XBB.1.5) in human serum. For the prototype strain, the MN50 assay met acceptance criteria for inter-/intra-assay precision, specificity, linearity, and selectivity. The assay was robust against changes to virus/serum incubation time, cell seeding density, virus content per well, cell passage number, and serum interference. Analyte in serum samples was stable up to five freeze/thaw cycles and for up to 12 months of storage at –80 ± 10 °C. Similar results were observed for the variant-adapted MN50 assays. The conversion factor to convert assay result units to WHO international standard units (IU/mL) was determined to be 0.62 for the prototype strain. This MN50 assay will be useful for vaccine immunogenicity analyses in clinical trial samples, enabling assessment of vaccine immunogenicity for ancestral and variant strains as variant-adapted vaccines are developed. •The microneutralization (MN50) assay is validated for multiple SARS-COV-2 strains.•The MN assay is calibrated to WHO international unit (NIBSC) standard.•This assay is suitable for testing clinical samples.•MN assay correlates with anti-rS IgG and hACE2 receptor binding inhibition assays.•The validated MN50 assay is useful to test vaccine immunogenicity.

Weiterführende Literatur

Empfehlungen zum selben Thema automatisch vorgeschlagen von bX