Details

Autor(en) / Beteiligte

• Dogan, Hamide
• Ozcimen, A. Ata
• Silici, Sibel

Titel

Interaction of olive oil-based propolis and caffeic acid phenethyl ester with methylprednisolone used in the treatment of human acute myeloid leukemia

Ist Teil von

• Molecular biology reports, 2024-12, Vol.51 (1), p.559-559, Article 559

Ort / Verlag

Dordrecht: Springer Netherlands

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• Background Methylprednisolone (MP) is a pharmaceutical agent employed in the management of Leukemia, which is a systemic malignancy that arises from abnormalities in the hematological system. Numerous investigations in the field of cancer research have directed their attention towards propolis, a natural substance with significant potential as a treatment-supportive agent. Its utilization aims to mitigate the potential adverse effects associated with chemotherapy medications. The objective of this study was to examine the impact of olive oil-based propolis (OEP) and caffeic acid phenethyl ester (CAPE) on the treatment of acute myeloid leukemia, as well as to determine if they exhibit a synergistic effect when combined with the therapeutic support product methylprednisolone. Methods and results The proliferation of HL-60 cells was quantified using the WST-8 kit. The PI Staining technique was employed to do cell cycle analysis of DNA in cells subjected to OEP, CAPE, and MP, with subsequent measurement by flow cytometry. The apoptotic status of cells was determined by analyzing them using flow cytometry after staining with the Annexin V-APC kit. The quantification of apoptotic gene expression levels was conducted in HL-60 cells. In HL-60 cells, the IC 50 dosages of CAPE and MP were determined to be 1 × 10 − 6 M and 5 × 10 − 4 M, respectively. The HL-60 cells were subjected to apoptosis and halted in the G 0 /G 1 and G 2 /M phases of the cell cycle after being treated with MP, CAPE, and OEP. Conclusions Propolis and its constituents have the potential to serve as effective adjunctive therapies in chemotherapy.