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The detection of foodborne pathogenic bacteria is critical in preventing foodborne diseases. DNA-based electrochemical biosensors, with the merits of high sensitivity and short detection time, provide an effective detecting method for foodborne pathogens, attracting significant interest for the past few years. This review mainly describes the important research progress of DNA-based electrochemical biosensors for the detection of foodborne pathogenic bacteria through four perspectives: representative foodborne pathogens detection using electrochemical approaches, DNA immobilization strategies of aptamers, DNA-based signal amplification strategies used in electrochemical DNA sensors, and functional DNA used in electrochemical DNA sensors. Finally, perspectives and challenges are presented in this field. This review will contribute to DNA-based electrochemical biosensor in enhancing the nucleic acid signal amplification.
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•Review on DNA-based electrochemical biosensors for foodborne pathogenic bacteria detection.•DNA immobilization strategies for DNA-based electrochemical biosensors have been discussed.•DNA-based signal amplification strategies is used to improve the sensitivity of the DNA-based electrochemical biosensors.•Functional DNA used in electrochemical DNA sensors are highlighted.