Details

Autor(en) / Beteiligte

• Shipa, Ahmed M.E.
• Kahilo, Khaled A.
• Elshazly, Samir A.
• Taher, Ehab S.
• Nasr, Nasr E.
• Alotaibi, Badriyah S.
• Almadaly, Essam A.
• Assas, Mona
• Abdo, Walied
• Abouzed, Tarek K.
• Salem, Abdulati Elsanusi
• Kirci, Damla
• El-Seedi, Hesham R.
• Refaey, Mohamed S.
• Rizk, Nermin I.
• Shukry, Mustafa
• Dorghamm, Doaa A.

Titel

Protective effect of Petroselinum crispum methanolic extract against acrylamide-induced reproductive toxicity in male rats through NF-ĸB, kinesin, steroidogenesis pathways

Ist Teil von

• Reproductive toxicology (Elmsford, N.Y.), 2024-06, Vol.126, p.108586, Article 108586

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• This study examined the protective effects of a Petroselinum crispum (P. crispum) methanolic extract on reproductive dysfunction induced by acrylamide in male rats. A total of 40 rats were divided into four groups (n=10). The control group received distilled water, the acrylamide group received 10 mg/kg of acrylamide, the P. crispum group received 100 mg/kg of P. crispum extract, and the combined group was pretreated with P. crispum for two weeks before co-administration of P. crispum and acrylamide. All administrations were administered orally using a gastric tube for eight weeks. Acrylamide decreased testosterone levels but did not affect levels of FSH or LH. It also increased testicular levels of (MDA) malondialdehyde and reduced activity of (SOD) superoxide dismutase and impairment of sperm parameters. Furthermore, the administration of acrylamide resulted in an elevation of tumor necrosis factor-alpha (TNF-α) levels and a reduction in the levels of steroidogenic acute regulatory protein (STAR) and cytochrome P450scc (P450scc). Acrylamide negatively affected the histopathological outcomes, Johnsen's score, the diameter of seminiferous tubules, and the thickness of the germinal epithelium. It also upregulated the expression of NF-ĸB P65 and downregulated the expression of kinesin motor protein. In contrast, treatment with P. crispum extract restored the levels of antioxidant enzymes, improved sperm parameters, and normalized the gene expression of TNF-α, IL-10, IL-6, iNOS, NF-ĸB, STAR, CYP17A1, 17β-HSD and P450scc. It also recovered testicular histological parameters and immunoexpression of NF-ĸB P65 and kinesin altered by acrylamide. P. crispum showed protective effects against acrylamide-induced reproductive toxicity by suppressing oxidative damage and inflammatory pathways. [Display omitted] •P. crispum extract protects against acrylamide-induced reproductive dysfunction in rats.•It suppresses oxidative damage and inflammation caused by acrylamide exposure.•P. crispum extract improves sperm parameters and restores antioxidant enzyme levels.•It normalizes gene expression of TNF-α, STAR, and P450scc in the testes.•P. crispum extract reverses histopathological damage and immunoexpression alterations.