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Autor(en) / Beteiligte
Titel
DNA barcoding, phylogeography and evolutionary dynamics of Chrysichthys auratus
Ist Teil von
  • Gene, 2024-07, Vol.917, p.148448-148448, Article 148448
Ort / Verlag
Netherlands: Elsevier B.V
Erscheinungsjahr
2024
Link zum Volltext
Quelle
Elsevier ScienceDirect Journals Complete
Beschreibungen/Notizen
  • •Chrysichthys auratus: Cylindrical body, ventrally flattened for benthic feeding. Barbels on nose, mouth sides, and chin.•Dorsal fins: First part with 2 spines, second part with 6–7 rays. Pectoral fins relatively long, with 1 spine and 7–8 rays.•Morphometrics: Total length 21.3–23.7 cm, standard length 81.94–82.70 %. Head length 31.72–35.20 % of standard length.•Molecular analysis: PCR amplification products resolved on 1% agarose gel. Evolutionary history inferred by Neighbor-Joining method.•Haplotype network: Minimum spanning network constructed to show haplotype distribution. Pi-value: 0.255278, Tajima's D: −0.448891. This study embarked on an exploration into the genetic structure and evolutionary history of the Chrysichthys auratus species, leveraging PCR amplification, phylogenetic trees, and haplotype networks. Specific DNA segments were successfully amplified and visualized through electrophoresis. Newly obtained sequences were Bank into GenBank and given accession numbers (OR730807-OR730808-OR730809). The Neighbor-Joining method provided insights into the evolutionary relationships among taxa, further augmented by bootstrap values and the Tamura 3-parameter method. A comprehensive geographical haplotype network showcased pronounced genetic differentiation, especially between remote populations. Nonetheless, shared haplotypes between proximate regions indicated either ancestral genetic connections or ongoing gene flow. Employing the COI-DNA barcodes, an in-depth understanding of intra- and inter-populational genetic diversity was achieved. The study's findings unravel the intricate genetic landscape and evolutionary dynamics of C. auratus, offering novel perspectives into its demographic history across its vast native habitat.
Sprache
Englisch
Identifikatoren
ISSN: 0378-1119
eISSN: 1879-0038
DOI: 10.1016/j.gene.2024.148448
Titel-ID: cdi_proquest_miscellaneous_3034774080

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