Details

Autor(en) / Beteiligte

• Jones, Amy G.
• Aquilino, Matilde
• Tinker, Rory J.
• Duncan, Laura
• Jenkins, Zandra
• Carvill, Gemma L.
• DeWard, Stephanie J.
• Grange, Dorothy K.
• Hajianpour, MJ
• Halliday, Benjamin J.
• Holder-Espinasse, Muriel
• Horvath, Judit
• Maitz, Silvia
• Nigro, Vincenzo
• Morleo, Manuela
• Paul, Victoria
• Spencer, Careni
• Esterhuizen, Alina I.
• Polster, Tilman
• Spano, Alice
• Gómez-Lozano, Inés
• Kumar, Abhishek
• Poke, Gemma
• Phillips, John A.
• Underhill, Hunter R.
• Gimenez, Gregory
• Namba, Takashi
• Robertson, Stephen P.

Titel

Clustered de novo start-loss variants in GLUL result in a developmental and epileptic encephalopathy via stabilization of glutamine synthetase

Ist Teil von

• American journal of human genetics, 2024-04, Vol.111 (4), p.729-741

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2024

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Glutamine synthetase (GS), encoded by GLUL, catalyzes the conversion of glutamate to glutamine. GS is pivotal for the generation of the neurotransmitters glutamate and gamma-aminobutyric acid and is the primary mechanism of ammonia detoxification in the brain. GS levels are regulated post-translationally by an N-terminal degron that enables the ubiquitin-mediated degradation of GS in a glutamine-induced manner. GS deficiency in humans is known to lead to neurological defects and death in infancy, yet how dysregulation of the degron-mediated control of GS levels might affect neurodevelopment is unknown. We ascertained nine individuals with severe developmental delay, seizures, and white matter abnormalities but normal plasma and cerebrospinal fluid biochemistry with de novo variants in GLUL. Seven out of nine were start-loss variants and two out of nine disrupted 5′ UTR splicing resulting in splice exclusion of the initiation codon. Using transfection-based expression systems and mass spectrometry, these variants were shown to lead to translation initiation of GS from methionine 18, downstream of the N-terminal degron motif, resulting in a protein that is stable and enzymatically competent but insensitive to negative feedback by glutamine. Analysis of human single-cell transcriptomes demonstrated that GLUL is widely expressed in neuro- and glial-progenitor cells and mature astrocytes but not in post-mitotic neurons. One individual with a start-loss GLUL variant demonstrated periventricular nodular heterotopia, a neuronal migration disorder, yet overexpression of stabilized GS in mice using in utero electroporation demonstrated no migratory deficits. These findings underline the importance of tight regulation of glutamine metabolism during neurodevelopment in humans. [Display omitted] Start-loss variants in GLUL result in a severe developmental and epileptic phenotype through the stabilization of glutamine synthetase, an enzyme crucial for brain function. This disorder is unusual in its gain-of-stabilization mechanism contrasting with the allelic deficiency disorder.