Details

Autor(en) / Beteiligte

• Jia, Wei-Li
• Zhang, Min
• Gao, Fang-Zhou
• Bai, Hong
• He, Lu-Xi
• He, Liang-Ying
• Liu, Ting
• Han, Yu
• Ying, Guang-Guo

Titel

Antibiotic resistome in landfill leachate and impact on groundwater

Ist Teil von

• The Science of the total environment, 2024-06, Vol.927, p.171991-171991, Article 171991

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2024

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Landfill leachate is a hotspot in antibiotic resistance development. However, little is known about antibiotic resistome and host pathogens in leachate and their effects on surrounding groundwater. Here, metagenomic sequencing was used to explore profiles, host bacteria, environmental risks and influencing factors of antibiotic resistome in raw and treated leachate and surrounding groundwater of three landfills. Results showed detection of a total of 324 antibiotic resistance genes (ARGs). The ARGs conferring resistance to multidrug (8.8 %–25.7 %), aminoglycoside (13.1 %–39.2 %), sulfonamide (10.0 %–20.9 %), tetracycline (5.7 %–34.4 %) and macrolide-lincosamide-streptogramin (MLS, 5.3 %–29.5 %) were dominant in raw leachate, while multidrug resistance genes were the major ARGs in treated leachate (64.1 %–83.0 %) and groundwater (28.7 %–76.6 %). Source tracking analysis suggests non-negligible influence of leachate on the ARGs in groundwater. The pathogens including Acinetobacter pittii, Pseudomonas stutzeri and P. alcaligenes were the major ARG-carrying hosts. Variance partitioning analysis indicates that the microbial community, abiotic variables and their interaction contributed most to the antibiotic resistance development. Our results shed light on the dissemination and driving mechanisms of ARGs from leachate to the groundwater, indicating that a comprehensive risk assessment and efficient treatment approaches are needed to deal with ARGs in landfill leachate and nearby groundwater. Antibiotic resistance genes are found abundant in the landfill sites, and these genes could be disseminated into groundwater via leaching of wastewater and infiltration of leachate. This results in deterioration of groundwater quality and human health risks posed by these ARGs and related pathogens. Thus measures should be taken to minimize potential negative impacts of landfills on the surrounding environment. [Display omitted] •17 ARG types and 324 ARG subtypes were detected in leachate and groundwater.•Multidrug, aminoglycoside, sulfonamide, tetracycline and MLS resistance genes were frequently detected.•pittii, P. stutzeri and P. alcaligenes were major host pathogens.•Leachate was a major source of ARGs in landfill surrounding groundwater.•Microbial community and abiotic variables were main driven factors altering ARGs.