UNIVERSI
TÄ
TS-
BIBLIOTHEK
P
ADERBORN
Anmelden
Menü
Menü
Start
Hilfe
Blog
Weitere Dienste
Neuerwerbungslisten
Fachsystematik Bücher
Erwerbungsvorschlag
Bestellung aus dem Magazin
Fernleihe
Einstellungen
Sprache
Deutsch
Deutsch
Englisch
Farbschema
Hell
Dunkel
Automatisch
Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist
gegebenenfalls
nur via VPN oder Shibboleth (DFN-AAI) möglich.
mehr Informationen...
Universitätsbibliothek
Katalog
Suche
Details
Zur Ergebnisliste
Ergebnis 3 von 542
Datensatz exportieren als...
BibTeX
UPK3A+ umbrella cell damage mediated by TLR3–NR2F6 triggers programmed destruction of urothelium in Hunner‐type interstitial cystitis/painful bladder syndrome
The Journal of pathology, 2024-06, Vol.263 (2), p.203-216
Peng, Liao
Chen, Jia‐Wei
Chen, Yuan‐Zhuo
Zhang, Chi
Shen, Si‐Hong
Liu, Meng‐Zhu
Fan, Yang
Yang, Shi‐Qin
Zhang, Xiu‐Zhen
Wang, Wei
Gao, Xiao‐Shuai
Di, Xing‐Peng
Ma, Yu‐Cheng
Zeng, Xiao
Shen, Hong
Jin, Xi
Luo, De‐Yi
2024
Details
Autor(en) / Beteiligte
Peng, Liao
Chen, Jia‐Wei
Chen, Yuan‐Zhuo
Zhang, Chi
Shen, Si‐Hong
Liu, Meng‐Zhu
Fan, Yang
Yang, Shi‐Qin
Zhang, Xiu‐Zhen
Wang, Wei
Gao, Xiao‐Shuai
Di, Xing‐Peng
Ma, Yu‐Cheng
Zeng, Xiao
Shen, Hong
Jin, Xi
Luo, De‐Yi
Titel
UPK3A+ umbrella cell damage mediated by TLR3–NR2F6 triggers programmed destruction of urothelium in Hunner‐type interstitial cystitis/painful bladder syndrome
Ist Teil von
The Journal of pathology, 2024-06, Vol.263 (2), p.203-216
Ort / Verlag
Chichester, UK: John Wiley & Sons, Ltd
Erscheinungsjahr
2024
Link zum Volltext
Quelle
Access via Wiley Online Library
Beschreibungen/Notizen
Urothelial damage and barrier dysfunction emerge as the foremost mechanisms in Hunner‐type interstitial cystitis/bladder pain syndrome (HIC). Although treatments aimed at urothelial regeneration and repair have been employed, their therapeutic effectiveness remains limited due to the inadequate understanding of specific cell types involved in damage and the lack of specific molecular targets within these mechanisms. Therefore, we harnessed single‐cell RNA sequencing to elucidate the heterogeneity and developmental trajectory of urothelial cells within HIC bladders. Through reclustering, we identified eight distinct clusters of urothelial cells. There was a significant reduction in UPK3A+ umbrella cells and a simultaneous increase in progenitor‐like pluripotent cells (PPCs) within the HIC bladder. Pseudotime analysis of the urothelial cells in the HIC bladder revealed that cells faced challenges in differentiating into UPK3A+ umbrella cells, while PPCs exhibited substantial proliferation to compensate for the loss of UPK3A+ umbrella cells. The urothelium in HIC remains unrepaired, despite the substantial proliferation of PPCs. Thus, we propose that inhibiting the pivotal signaling pathways responsible for the injury to UPK3A+ umbrella cells is paramount for restoring the urothelial barrier and alleviating lower urinary tract symptoms in HIC patients. Subsequently, we identified key molecular pathways (TLR3 and NR2F6) associated with the injury of UPK3A+ umbrella cells in HIC urothelium. Finally, we conducted in vitro and in vivo experiments to confirm the potential of the TLR3–NR2F6 axis as a promising therapeutic target for HIC. These findings hold the potential to inhibit urothelial injury, providing promising clues for early diagnosis and functional bladder self‐repair strategies for HIC patients. © 2024 The Pathological Society of Great Britain and Ireland.
Sprache
Englisch
Identifikatoren
ISSN: 0022-3417
eISSN: 1096-9896
DOI: 10.1002/path.6275
Titel-ID: cdi_proquest_miscellaneous_3022570518
Format
–
Schlagworte
Animals
,
Bladder
,
Cell Differentiation
,
Cell Proliferation
,
Cystitis
,
Cystitis, Interstitial - genetics
,
Cystitis, Interstitial - metabolism
,
Cystitis, Interstitial - pathology
,
Female
,
Humans
,
Hunner‐type interstitial cystitis/bladder pain syndrome
,
Male
,
NR2F6
,
Pluripotency
,
Progenitor cells
,
Signal Transduction
,
Single-Cell Analysis
,
single‐cell RNA sequencing
,
Therapeutic targets
,
TLR3
,
TLR3 protein
,
Toll-Like Receptor 3 - genetics
,
Toll-Like Receptor 3 - metabolism
,
Toll-like receptors
,
Urinary Bladder - metabolism
,
Urinary Bladder - pathology
,
Urinary tract
,
Urothelium
,
Urothelium - metabolism
,
Urothelium - pathology
Weiterführende Literatur
Empfehlungen zum selben Thema automatisch vorgeschlagen von
bX