Details

Autor(en) / Beteiligte

• Yu, Qian
• Shu, Shi
• Ju, Xin-yao
• Peng, Wei
• Ren, Xue-qi
• Si, Shu-han
• Song, Shi-zhen
• Xie, Xue-yun
• Fang, Bang-jiang
• Zhou, Shuang

Titel

Electroacupuncture Promotes Angiogenesis in Mice with Cerebral Ischemia by Inhibiting miR-7

Ist Teil von

• Chinese journal of integrative medicine, 2024-06, Vol.30 (6), p.543-550

Ort / Verlag

Singapore: Springer Nature Singapore

Erscheinungsjahr

2024

Link zum Volltext

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• Objective To observe the angiogenesis effect of electroacupuncture (EA) at Shuigou acupoint (GV 26) in the treatment of cerebral ischemia, and explore the value of miRNA-7 (miR-7) in it. Methods First, 48 mice were randomly divided into sham operation, middle cerebral artery occlusion (MCAO) model, and EA treatment groups. Then 9 mice were divided into carrier control group, miR-7 knockout group and miR-7 overexpression group ( n =3 each group). Finally, 20 mice were divided into model and carrier control group, model and miR-7 knockout group, EA treatment and carrier control group and EA treatment and miR-7 overexpression group, with 3–6 mice in each group. The MCAO model was established in the MCAO and EA groups. Neurological deficit score and 2,3,5-triphenyltetrazolium chloride (TTC) staining were used to evaluate the severity of cerebral ischemia. Hematoxylin-eosin staining was used to describe basic pathological changes. Immunohistochemistry was used to quantify cerebral microvessel density. Real-time PCR and Western blot were used to detect the expression of miR-7 and its downstream target genes Krüppel-like factor 4/vascular endothelial growth factor (KLF4/VEGF) and angiopoietin-2 (ANG-2) in the ischemic cerebral cortex. Results After EA, neurological deficit scores and infarction volumes decreased, and the density of cerebral microvessels increased. In the MCAO group, miR-7 expression was higher than that in the sham group ( P <0.01). After EA at GV 26, miR-7 expression decreased ( P <0.01) and the expression of downstream target genes KLF4/VEGF and ANG-2 increased as compared with the MCAO group ( P <0.01). After EA combined with overexpression of miR-7, the expression of downstream target genes KLF4/VEGF and ANG-2 decreased compared to the control EA group ( P <0.01). After miR-7 knockdown, the expression of KLF4/VEGF and ANG-2 increased ( P <0.05 or P <0.01). Conclusions EA could promote angiogenesis in MCAO mice likely by inhibiting the expression of miR-7 and relieving inhibition of downstream target genes KLF4/VEGF and ANG-2.