Details

Autor(en) / Beteiligte

• Fu, Dong-wen
• Xu, Hang
• Sun, Rui-qi
• Liu, Xue-li
• Ji, Zhe
• Zhou, Da-yong
• Song, Liang

Titel

Engineering marine phospholipid nanoliposomes via glycerol-infused proliposomes: Mechanisms, strategies, and versatile applications in scalable food-grade nanoliposome production

Ist Teil von

• Food chemistry, 2024-08, Vol.448, p.139030-139030, Article 139030

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• [Display omitted] •Solvent-free and scalable food-grade KOP proliposomes are formulated with glycerol.•Glycerol in proliposomes unpacked the stacked chain acyl of phospholipids.•Hydration of proliposomes yielded stable multilayer nanoliposomes.•Nanoliposome formation remained stable during storage and environmental stresses.•Proliposomes enhanced in vitro bioaccessibility of phospholipids. This study presents a novel approach using polyol-based proliposome to produce marine phospholipids nanoliposomes. Proliposomes were formulated by blending glycerol with phospholipids across varying mass ratios (2:1 to 1:10) at room temperature. Analysis employing polarized light microscopy, FTIR, and DSC revealed that glycerol disrupted the stacked acyl groups within phospholipids, lowering the phase transition temperature (Tm). Krill oil phospholipids (KOP) proliposomes exhibited superior performance in nanoliposomes formation, with a mean diameter of 125.60 ± 3.97 nm, attributed to the decreased Tm (−7.64 and 7.00 °C) compared to soybean phospholipids, along with a correspondingly higher absolute zeta potential (−39.77 ± 1.18 mV). The resulting KOP proliposomes demonstrated liposomes formation stability over six months and under various environmental stresses (dilution, thermal, ionic strength, pH), coupled with in vitro absorption exceeding 90 %. This investigation elucidates the mechanism behind glycerol-formulated proliposomes and proposes innovative strategies for scalable, solvent-free nanoliposome production with implications for functional foods and pharmaceutical applications.