Details

Autor(en) / Beteiligte

• Liu, Xuanbei
• Hong, Enhui
• Xie, Jiayu
• Li, Jiangwei
• Ding, Boyun
• Chen, Yongsheng
• Xia, Zhennan
• Jiang, Weiping
• Lv, Hongzhu
• Yang, Bo
• Chen, Yizhao

Titel

Txnrd2 Attenuates Early Brain Injury by Inhibition of Oxidative Stress and Endoplasmic Reticulum Stress via Trx2/Prx3 Pathway after Intracerebral Hemorrhage in Rats

Ist Teil von

• Neuroscience, 2024-05, Vol.545, p.158-170

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2024

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• [Display omitted] •Txnrd2 may be involved in brain injury caused by intracerebral hemorrhage (ICH)•Txnrd2 increased in neurons, astrocytes cells post-ICH.•Txnrd2 knockdown increased the levels of oxidative stress and ER-stress in the brain post-ICH.•Se may partly alleviate the early brain injury after ICH caused by oxidative stress and ER stress via Txnrd2.•Txnrd2 may represent a therapeutic target for ICH. Thioredoxin-reductase 2 (Txnrd2) belongs to the thioredoxin-reductase family of selenoproteins and is a key antioxidant enzyme in mammalian cells to regulate redox homeostasis. Here, we reported that Txnrd2 exerted a major influence in brain damage caused by Intracerebral hemorrhage (ICH) by suppressing endoplasmic reticulum (ER) stress oxidative stress and via Trx2/Prx3 pathway. Furthermore, we demonstrated that pharmacological selenium (Se) rescued the brain damage after ICH by enhancing Txnrd2 expression. Primarily, expression and localization of Txnrd2, Trx2 and Prx3 were determined in collagenase IV-induced ICH model. Txnrd2 was then knocked down using siRNA interference in rats which were found to develop more severe encephaledema and neurological deficits. Mechanistically, we observed that loss of Txnrd2 leads to increased lipid peroxidation levels and ER stress protein expression in neurons and astrocytes. Additionally, it was revealed that Se effectively restored the expression of Txnrd2 in brain and inhibited both the activity of ER stress protein activity and the generation of reactive oxygen species (ROS) by promoting Trx2/Prx3 kilter when administrating sodium selenite in lateral ventricle. This study shed light on the effect of Txnrd2 in regulating oxidative stress and ER stress via Trx2/Prx3 pathway upon ICH and its promising potential as an ICH therapeutic target.