Details

Autor(en) / Beteiligte

• Tang, Qian
• Ojiro, Ryota
• Ozawa, Shunsuke
• Zou, Xinyu
• Nakahara, Junta
• Nakao, Tomohiro
• Koyanagi, Mihoko
• Jin, Meilan
• Yoshida, Toshinori
• Shibutani, Makoto

Titel

DNA methylation-altered genes in the rat hippocampal neurogenic niche after continuous exposure to amorphous curcumin

Ist Teil von

• Journal of chemical neuroanatomy, 2024-04, Vol.137, p.102414-102414, Article 102414

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2024

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Rat offspring who are exposed to an amorphous formula of curcumin (CUR) from the embryonic stage have anti-anxiety-like behaviors, enhanced fear extinction learning, and increased synaptic plasticity in the hippocampal dentate gyrus (DG). In the present study, we investigated the links between genes with altered methylation status in the neurogenic niche and enhanced neural functions after CUR exposure. We conducted methylation and RNA sequencing analyses of the DG of CUR-exposed rat offspring on day 77 after delivery. Methylation status and transcript levels of candidate genes were validated using methylation-sensitive high-resolution melting and real-time reverse-transcription PCR, respectively. In the CUR group, we confirmed the hypermethylation and downregulation of Gpr150, Mmp23, Rprml, and Pcdh8 as well as the hypomethylation and upregulation of Ppm1j, Fam222a, and Opn3. Immunohistochemically, reprimo-like+ hilar cells and protocadherin-8+ granule cells were decreased and opsin-3+ hilar cells were increased by CUR exposure. Both reprimo-like and opsin-3 were partially expressed on subpopulations of glutamic acid decarboxylase 67+ γ-aminobutyric acid-ergic interneurons. Furthermore, the transcript levels of genes involved in protocadherin-8-mediated N-cadherin endocytosis were altered with CUR exposure; this was accompanied by Ctnnb1 and Syp upregulation and Mapk14, Map2k3, and Grip1 downregulation, suggesting that CUR-induced enhanced synaptic plasticity is associated with cell adhesion. Together, our results indicate that functionally different genes have altered methylation and expression in different neuronal populations of the hippocampal neurogenic niche, thus enhancing synaptic plasticity after CUR exposure. •CUR-induced DNA methylation-altered genes were identified in the hippocampal DG.•Functionally different genes were confirmed to be up or downregulated by CUR.•Gpr150, Rprml, Pcdh8, and Opn3 showed neuron type-specific expression in the DG.•CUR decreased the synaptic plasticity-related protocadherin-8+ granule cell number.•CUR altered expression of protocadherin-8-mediated endocytosis-related genes.