Details

Autor(en) / Beteiligte

• Clemons, Rebecca A.
• Yacoub, Mark N.
• Faust, Evelyn
• Toledo, L. Felipe
• Jenkinson, Thomas S.
• Carvalho, Tamilie
• Simmons, D. Rabern
• Kalinka, Erik
• Fritz-Laylin, Lillian K.
• James, Timothy Y.
• Stajich, Jason E.

Titel

An endogenous DNA virus in an amphibian-killing fungus associated with pathogen genotype and virulence

Ist Teil von

• Current biology, 2024-04, Vol.34 (7), p.1469-1478.e6

Ort / Verlag

England: Elsevier Inc

Erscheinungsjahr

2024

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The global panzootic lineage (GPL) of the pathogenic fungus Batrachochytrium dendrobatidis (Bd) has caused severe amphibian population declines, yet the drivers underlying the high frequency of GPL in regions of amphibian decline are unclear. Using publicly available Bd genome sequences, we identified multiple non-GPL Bd isolates that contain a circular Rep-encoding single-stranded (CRESS)-like DNA virus, which we named Bd DNA virus 1 (BdDV-1). We further sequenced and constructed genome assemblies with long read sequences to find that the virus is integrated into the nuclear genome in some strains. Attempts to cure virus-positive isolates were unsuccessful; however, phenotypic differences between naturally virus-positive and virus-negative Bd isolates suggested that BdDV-1 decreases the growth of its host in vitro but increases the virulence of its host in vivo. BdDV-1 is the first-described CRESS DNA mycovirus of zoosporic true fungi, with a distribution inversely associated with the emergence of the panzootic lineage. •BdDV-1 is a novel DNA virus of the amphibian pathogen Batrachochytrium dendrobatidis•BdDV-1 is endogenous in the genome but absent from global panzootic lineage strains•Strains with endogenized virus have reduced in vitro growth but increased virulence•BdDV-1 is the first full-length CRESS virus of Circoviridae known to infect fungi Clemons and Yacoub et al. describe a novel CRESS DNA virus, BdDV-1, of the amphibian pathogen Batrachochytrium dendrobatidis. BdDV-1 is endogenized in the fungal genome and found primarily within less-virulent, non-GPL lineages of the fungus. BdDV-1 presence is associated with reduced in vitro growth and increased virulence in an amphibian model.