Details

Autor(en) / Beteiligte

• Rode, Surabhi
• Kaur, Harry
• Sharma, Monica
• Shah, Vivek
• Singh, Shiv Shakti
• Gubyad, Mrugendra
• Ghosh, Dilip Kumar
• Sircar, Debabrata
• Kumar, Pravindra
• Roy, Partha
• Sharma, Ashwani Kumar

Titel

Characterization of Type1 Lipid Transfer Protein from Citrus sinensis: Unraveling its potential as an antimicrobial and insecticidal agent

Ist Teil von

• International journal of biological macromolecules, 2024-04, Vol.265 (Pt 1), p.130811-130811, Article 130811

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2024

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• Lipid Transfer Protein1 (LTP1) is a cationic, multifaceted protein belonging to the pathogenesis-related protein (PR14) family. Despite being involved in diverse physiological processes and defense mechanisms, the precise in-vivo role of LTP1 remains undiscovered. This work presents the characterization of recombinant Citrus sinensis LTP1 (CsLTP1) along with lipid binding studies through in-silico and in-vitro approaches. CsLTP1 demonstrated great thermal and pH stability with a huge biotechnological potential. It showed in-vitro binding capacity with jasmonic acid and lipids involved in regulating plant immune responses. Gene expression profiling indicated a significant upregulation of CsLTP1 in Candidatus-infected Citrus plants. CsLTP1 disrupted the cell membrane integrity of various pathogens, making it a potent antimicrobial agent. Further, in-vivo antimicrobial and insecticidal properties of CsLTP1 have been explored. The impact of exogenous CsLTP1 treatment on rice crop metabolism for managing blight disease has been studied using GC–MS. CsLTP1 triggered crucial metabolic pathways in rice plants while controlling the blight disease. CsLTP1 effectively inhibited Helicoverpa armigera larvae by impeding mid-gut α-amylase activity and obstructing its developmental stages. This study highlights the pivotal role of CsLTP1 in plant defense by offering insights for developing multi-target therapeutic agent or disease-resistant varieties to comprehensively tackle the challenges towards crop protection. •Type1 LTP with α-Amylase inhibitory activity was characterized from Citrus sinensis.•It possesses a higher binding affinity towards unsaturated lipid molecules.•CsLTP1 displayed a strong antimicrobial activity in in-vitro and in-planta studies.•It activated crucial metabolic pathways in rice plants to control bacterial blight.•CsLTP1 exhibits potent insecticidal activity against Helicoverpa armigera.