Details

Autor(en) / Beteiligte

• Joulia, Régis
• Puttur, Franz
• Stölting, Helen
• Traves, William J
• Entwistle, Lewis J
• Voitovich, Anastasia
• Martin, Minerva Garcia
• Al-Sahaf, May
• Bonner, Katie
• Scotney, Elizabeth
• Molyneaux, Philip L
• Hewitt, Richard J
• Walker, Simone A
• Yates, Laura
• Saglani, Sejal
• Lloyd, Clare M

Titel

Mast cell activation disrupts interactions between endothelial cells and pericytes during early life allergic asthma

Ist Teil von

• The Journal of clinical investigation, 2024-03, Vol.134 (6), p.1-15

Ort / Verlag

Ann Arbor: American Society for Clinical Investigation

Erscheinungsjahr

2024

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Allergic asthma generally starts during early life and is linked to substantial tissue remodeling and lung dysfunction. Although angiogenesis is a feature of the disrupted airway, the impact of allergic asthma on the pulmonary microcirculation during early life is unknown. Here, using quantitative imaging in precision-cut lung slices (PCLSs), we report that exposure of neonatal mice to house dust mite (HDM) extract disrupts endothelial cell/pericyte interactions in adventitial areas. Central to the blood vessel structure, the loss of pericyte coverage was driven by mast cell (MC) proteases, such as tryptase, that can induce pericyte retraction and loss of the critical adhesion molecule N-cadherin. Furthermore, spatial transcriptomics of pediatric asthmatic endobronchial biopsies suggests intense vascular stress and remodeling linked with increased expression of MC activation pathways in regions enriched in blood vessels. These data provide previously unappreciated insights into the pathophysiology of allergic asthma with potential long-term vascular defects.