Details

Autor(en) / Beteiligte

• Loo, Eliza P.-I.
• Durán, Paloma
• Pang, Tin Yau
• Westhoff, Philipp
• Deng, Chen
• Durán, Carlos
• Lercher, Martin
• Garrido-Oter, Ruben
• Frommer, Wolf B.

Titel

Sugar transporters spatially organize microbiota colonization along the longitudinal root axis of Arabidopsis

Ist Teil von

• Cell host & microbe, 2024-04, Vol.32 (4), p.543-556.e6

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2024

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Plant roots are functionally heterogeneous in cellular architecture, transcriptome profile, metabolic state, and microbial immunity. We hypothesized that axial differentiation may also impact spatial colonization by root microbiota along the root axis. We developed two growth systems, ArtSoil and CD-Rhizotron, to grow and then dissect Arabidopsis thaliana roots into three segments. We demonstrate that distinct endospheric and rhizosphere bacterial communities colonize the segments, supporting the hypothesis of microbiota differentiation along the axis. Root metabolite profiling of each segment reveals differential metabolite enrichment and specificity. Bioinformatic analyses and GUS histochemistry indicate microbe-induced accumulation of SWEET2, 4, and 12 sugar uniporters. Profiling of root segments from sweet mutants shows altered spatial metabolic profiles and reorganization of endospheric root microbiota. This work reveals the interdependency between root metabolites and microbial colonization and the contribution of SWEETs to spatial diversity and stability of microbial ecosystem. [Display omitted] •Root microbiota, metabolites, and SWEET uniporters are patterned along the root axis•SWEET sugar uniporter organization correlates with sugar metabolism pathways in the root•Root metabolites and microbiota are spatially reorganized in sweet-deficient plants•SWEET2, -4, and -11;12 play a functional role in spatial colonization of microbiota Loo et al. develop two growth systems to define the spatial organization of microbiota and metabolites along the longitudinal axis of the Arabidopsis thaliana root. SWEET sugar transporters contribute to the distribution of sugar (and other metabolites) along the root, which is required for spatial colonization of root bacteria.