Details

Autor(en) / Beteiligte

• Liang, Pingzhuo
• Guo, Mingyu
• Wang, Dan
• Li, Ting
• Li, Ren
• Li, Dapeng
• Cheng, Shenhang
• Zhen, Congai
• Zhang, Lei

Titel

Molecular and functional characterization of heat-shock protein 70 in Aphis gossypii under thermal and xenobiotic stresses

Ist Teil von

• Pesticide biochemistry and physiology, 2024-02, Vol.199, p.105774-105774, Article 105774

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2024

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• Aphis gossypii, a globally distributed and economically significant pest of several crops, is known to infest a wide range of host plants. Heat shock proteins (Hsps), acting as molecular chaperones, are essential for the insect's environmental stress responses. The present study investigated the molecular characteristics and expression patterns of AgHsp70, a heat shock protein gene, in Aphis gossypii. Our phylogenetic analysis revealed that AgHsp70 shared high similarity with homologs from other insects, suggesting a conserved function across species. The developmental expression profiles of AgHsp70 in A. gossypii showed that the highest transcript levels were observed in the fourth instar nymphs, while the lowest levels were detected in the third instar nymphs. Heat stress and exposure to four different xenobiotics (2-tridecanone, tannic acid, gossypol, and flupyradifurone (4-[(2,2-difluoroethyl)amino]-2(5H)-furanone)) significantly up-regulated AgHsp70 expression. Knockdown of AgHsp70 using RNAi obviously increased the susceptibility of cotton aphids to 2-tridecanone, gossypol and flupyradifurone. Dual-luciferase reporter assays revealed that gossypol and flupyradifurone significantly enhanced the promoter activity of AgHsp70 at a concentration of 10 mg/L. Furthermore, we identified the transcription factor heat shock factor (HSF) as a regulator of AgHsp70, as silencing AgHSF reduced AgHsp70 expression. Our results shed light on the role of AgHsp70 in xenobiotic adaptation and thermo-tolerance. [Display omitted] •One heat shock protein, named AgHsp70, was identified in the cotton aphid.•Under thermal stress, only heat significantly upregulated the AgHsp70 expression.•AgHsp70 was responsible to 2-tridecanone, gossypol and flupyradifurone.•The promoter activity was significantly enhanced by gossypol and flupyradifurone.•AgHsp70 was possibly regulated by AgHSF via RNAi method.