Details

Autor(en) / Beteiligte

• Abd-Elhakim, Yasmina M.
• Mohamed, Amany Abdel-Rahman
• Noreldin, Ahmed E.
• Khamis, Tarek
• Eskandrani, Areej A.
• Shamlan, Ghalia
• Alansari, Wafa S.
• Alotaibi, Badriyah S.
• Alosaimi, Manal E.
• Hakami, Mohammed Ageeli
• Abuzahrah, Samah S.

Titel

Fenpropathrin provoked kidney damage via controlling the NLRP3/Caspase-1/GSDMD–mediated pyroptosis: The palliative role of curcumin-loaded chitosan nanoparticles

Ist Teil von

• Toxicology and applied pharmacology, 2024-03, Vol.484, p.116869-116869, Article 116869

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2024

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• This study assessed the ability of formulated curcumin-loaded chitosan nanoparticles (CU-CS-NPs) to reduce the kidney damage resulting from fenpropathrin (FPN) in rats compared to curcumin (CU) in rats. Sixty male Sprague Dawley rats were separated into six groups and orally administered 1 mL/kg b.wt corn oil, 50 mg CU/kg b.wt, 50 mg CU-CS-NPs /kg b.wt., 15 mg FPN /kg b.wt, CU+ FPN or CU-CS-NPs + FPN for 60 days. Then, serum renal damage products were assessed. Total antioxidant capacity, reactive oxygen species, interleukin 1β (IL-1β), malondialdehyde, NF-κB P65, cleaved-Caspase-1, and Caspase-8 were estimated in kidney homogenates. The cleaved Caspase-3 and TNF-α immunoexpression and pyroptosis-related genes were determined in renal tissues. The results showed that CU-CS-NPS significantly repressed the FPN-induced increment in kidney damage products (urea, uric acid, and creatinine). Moreover, the FPN-associated hypo-proteinemia, renal oxidative stress and apoptotic reactions, and impaired renal histology were considerably repaired by CU and CU-CS-NPs. Additionally, compared to FPN-exposed rats, CU, and CU-CS-NPs-treated rats had considerably lower immunoexpression of cleaved Caspase-3 and TNF-α in renal tissue. The pyroptosis-related genes NLRP3, GSDMD, IL-18, Caspase-3, Caspase-1, IL-1β, Caspase-8, TNF-α, and NF-κB dramatically upregulated by FPN exposure in the renal tissues. Yet, in CU and CU-CS-NPs-treated rats, the gene above expression deviations were corrected. Notably, CU-CS-NPs were superior to CU in preventing oxidative damage and inflammation and regulating pyroptosis in the renal tissues of the FPN-exposed group. The results of the present study conclusively showed the superior favorable effect of CU-CS-NPs in counteracting renal impairment linked to environmental pollutants. [Display omitted] •The pyroptosis role in the kidney injury caused by fenpropathrin (FPN) was explored.•NLRP3/Caspase-1/GSDMD–mediated pyroptosis pathway was activated in FPN-exposed rats.•ROS generation was augmented in the FPN-exposed rats' kidney.•Curcumin-loaded chitosan nanoparticles nephroprotective activity surpassed curcumin.