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Leucaena leucocephala poisoning is reported in horses in different Brazilian regions. The poisoning occurred one month after the horses were introduced into paddocks invaded by the plant or after 10 days of consuming cut Leucaena administered as the only food. Affected horses showed moderate to severe hair loss on the mane and tail, orthokeratotic hyperkeratosis with marked follicular telogenization, and hyperplasia of thyroid follicular cells. Mimosin concentration in leaves (5.5 mg/g) was determined by a new HLPC-UV method which is also reported.
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•Leucaena leucocephala toxicity is rarely reported affecting horses.•The intoxication occurs in the dry season in many regions of Brazil.•Horses are affected when introduced into paddocks invaded by Leucaena and when they only eat after they have been cut.•Follicular telogenization is the most remarkable dermatological lesion.