Details

Autor(en) / Beteiligte

• Farias-Vazquez, Liliana S
• Ramos-González, Rodolfo
• Pacios-Michelena, Sandra
• Aguilar, Cristóbal N
• Arredondo-Valdés, Roberto
• Rodríguez-Herrera, Raúl
• Martínez-Hernández, José L
• Segura-Ceniceros, Elda P
• Ilyina, Anna

Titel

Antifungal Activity Enhancement of Cell-Free Streptomyces griseus Extract Obtained by Fermentation with Magnetic Manganese Ferrite Nanoparticles

Ist Teil von

• Applied biochemistry and biotechnology, 2024-01

Ort / Verlag

United States

Erscheinungsjahr

2024

Quelle

SpringerLink

Beschreibungen/Notizen

• The present study aims to obtain manganese ferrite nanoparticles functionalized with chitosan (C-MNP) or ethylenediamine (E-MNP) by coprecipitation and polyol one-step methods, characterize their interaction with S. griseus demonstrating cell immobilization, and evaluate the antimicrobial activity of the free cell extracts obtained from immobilized S. griseus fermentation in the presence of different concentrations of MNP. The adsorption isotherms were analyzed mathematically using Langmuir and Freundlich models. The highest coefficient of determination (R ) for the S. griseus cell adsorption isotherm with C-MNP was observed with a linear function of the Langmuir model. The adsorption isotherm of S. griseus cells with E-MNP was better fitted to the Freundlich model. Cell immobilization by adsorption on magnetic nanoparticles was demonstrated in both cases. Different concentrations of C-MNP and E-MNP were used in fermentations to prepare cell-free extracts with antifungal activity. The best results were obtained with E-MNP, with a 91.5% inhibition of radial fungal growth. Magnetic nanoparticles offer potential applications in different fields and easy biomass separation.