Details

Autor(en) / Beteiligte

• Juma, Kevin Maafu
• Morimoto, Kenta
• Sharma, Vishnu
• Sharma, Kirti
• Biyani, Radhika
• Biyani, Manish
• Takita, Teisuke
• Yasukawa, Kiyoshi

Titel

Detection of SARS-CoV-2 spike protein D614G mutation using μTGGE

Ist Teil von

• Molecular biology reports, 2024-12, Vol.51 (1), p.289-289, Article 289

Ort / Verlag

Dordrecht: Springer Netherlands

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• Background The accurate and expeditious detection of SARS-CoV-2 mutations is critical for monitoring viral evolution, assessing its impact on transmission, virulence, and vaccine efficacy, and formulating public health interventions. In this study, a detection system utilizing micro temperature gradient gel electrophoresis (μTGGE) was developed for the identification of the D614 and G614 variants of the SARS-CoV-2 spike protein. Methods The in vitro synthesized D614 and G614 gene fragments of the SARS-CoV-2 spike protein were amplified via polymerase chain reaction and subjected to μTGGE analysis. Results The migration patterns exhibited by the D614 and G614 variants on the polyacrylamide gel were distinctly dissimilar and readily discernible by μTGGE. In particular, the mid-melting pattern of D614 was shorter than that of G614. Conclusions Our results demonstrate the capability of μTGGE for the rapid, precise, and cost-effective detection of SARS-CoV-2 spike protein D614 and G614 variants without the need for sequencing. Therefore, this approach holds considerable potential for use in point-of-care mutation assays for SARS-CoV-2 and other pathogens.