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Effect of conditions of three dimensional clinostating on testicular cell machinery
Ist Teil von
Acta astronautica, 2007-02, Vol.60 (4), p.391-396
Ort / Verlag
Elsevier Ltd
Erscheinungsjahr
2007
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Our scope was to study the effects of on ground random positioning machine rotation on swine testicular cells in culture. Cells of the
2
n
karyotype line, from trypsinized swine testes, were submitted to modeled microgravity
(
μ
G
)
using a 3D RPM from 15
min to 24
h. The cultured cells were then fixed and submitted to immunohistochemistry using antibodies to steroid dehydrogenases, heat shock proteins and the sodium pump
(
Na
+
/
K
+
ATPase).
The results revealed that, after 15
min at modeled
μ
G
, all the cells showed damages at cytoskeletal level. The immunoreactions for
3
β
HSD
,
17
β
HSD
and
Na
+
/
K
+
ATPase were almost abolished. After 24
h of treatment, the presence of the enzymes was restored, and small heat shock proteins were strongly immunostainable. On the other hand, in the
1
×
G
cultures, the expression of HSPs was very weak.
We conclude that modeled
μ
G
by random positioning of cells affects testicular cells in culture for a short time, while the normal activity of the cells is restored after 24
h.