Details

Autor(en) / Beteiligte

• Ren, Xiaomeng
• Li, Kaiyi
• Li, Chunlei

Titel

Detection of molecular affecting sensitivity to local glucocorticoid therapy in oral lichen planus through transcriptome sequencing

Ist Teil von

• Beijing da xue xue bao. Journal of Peking University. Yi xue ban, 2024-02, Vol.56 (1), p.32-38

Ort / Verlag

China

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• To detect key genes of local glucocorticoid therapy in oral lichen planus (OLP) through transcriptome sequencing. The study prospectively enrolled 28 symptomatic patients who visitied Department of Oral Mucosa, Peking University Hospital of Stomatology from November 2019 to March 2023. Topical inunction of 0.1 g/L of dexamethasone was applied for 1 min, 3 times daily for 4 weeks. The patients' signs and pain symptoms were recorded and they were classified as effective group and ineffective group according to the treatment outcome. Their mucosa samples were collected before treatment. After isolating total RNA, transcriptome sequencing was performed. The gene expression data obtained by sequencing were analyzed differently using the DESeq2 package in R software, and the Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis was performed on the basis of the hypergeometric distribution algorithm to describe the biological function of differentially expressed genes (DEGs), accordingly detecti