Journal of micromechanics and microengineering, 2006-02, Vol.16 (2), p.425-433
2006
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Details
- Autor(en) / Beteiligte
- Titel
- DNA amplification on a PDMS–glass hybrid microchip
- Ist Teil von
- Journal of micromechanics and microengineering, 2006-02, Vol.16 (2), p.425-433
- Ort / Verlag
- Bristol: IOP Publishing
- Erscheinungsjahr
- 2006
- Quelle
- Alma/SFX Local Collection
- Beschreibungen/Notizen
- In this paper, the development of a hybrid polydimethylsiloxane (PDMS)-glass microchip for PCR (polymerase chain reaction) was reported. A novel temperature control strategy was applied for precise temperature control. The microchip was composed of two parts-a PDMS substrate and a glass cover. The PDMS substrate includes a reaction chamber for PCR and two microchannels for the inlet and the outlet of the sample. A glass cover integrating heaters and sensors was bonded to the PDMS substrate. The microchip was produced by molding PDMS against a microfabricated master with comparatively simple and inexpensive methods. PCR was successfully carried out on the PDMS-glass hybrid microchip with a 183 bp target of DNA.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0960-1317eISSN: 1361-6439DOI: 10.1088/0960-1317/16/2/029Titel-ID: cdi_proquest_miscellaneous_29202742
- Format
- –
- Schlagworte
- Applied fluid mechanics, Applied sciences, Biological and medical sciences, Biotechnology, Exact sciences and technology, Fluid dynamics, Fluidics, Fundamental and applied biological sciences. Psychology, Fundamental areas of phenomenology (including applications), Genetic engineering, Genetic technics, In vitro gene amplification. Pcr technique, Instruments, apparatus, components and techniques common to several branches of physics and astronomy, Mechanical engineering. Machine design, Mechanical instruments, equipment and techniques, Methods. Procedures. Technologies, Micromechanical devices and systems, Physics, Precision engineering, watch making