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Recovering rhamnogalacturonan-I pectin from sugar beet pulp using a sequential ultrasound and microwave-assisted extraction: Study on extraction optimization and membrane purification
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•Efficient RG-I pectin recovery with sequential UAE/MAE and membrane purification.•Use of short-time UAE decreased MAE time and temperature.•Optimal recovery of 66.0 % of pectooligosaccharides from sugar beet pulp.•The 90 % of POS were recovered using continuous DF and UF with 3 kDa MWCO membrane.•High-purity RG-I pectin with high-methoxyl attributes similar to prebiotics.
This research focuses on the integrated recovery of rhamnogalacturonan-I (RG-I) pectin from sugar beet pulp (SBP). First, the extraction of RG-I pectin through sequential ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) was assessed. Optimization using a response surface methodology identified the optimal conditions as initial pH 4, 10 min of UAE, and 157 °C for MAE, achieving a 66.0 % recovery of pectooligosaccharides (POS). Additionally, purification through continuous diafiltration and concentration via ultrafiltration of the POS using membranes with different molecular weight cut-offs (MWCO) was explored. In contrast to previous research using discontinuous diafiltration, the use of continuous diafiltration allowed a decrease in the extract viscosity and obtained higher yields using a higher MWCO membrane. The refined RG-I pectin solids exhibited a high global yield (39–40 g pectin/100 g SBP), and high-methoxyl characteristics, as well as purity levels (70–80 %) similar to commercial prebiotics.