Details

Autor(en) / Beteiligte

• Zheng, Jiancheng
• Zhao, Jian
• Li, Cuidi
• Zhang, Fangke
• Saiding, Qimanguli
• Zhang, Xingkai
• Wang, Guojun
• Qi, Jin
• Cui, Wenguo
• Deng, Lianfu

Titel

Targeted Protein Fate Modulating Functional Microunits Promotes Intervertebral Fusion

Ist Teil von

• Small methods, 2024-08, Vol.8 (8), p.e2301375-n/a

Ort / Verlag

Germany

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• Stable regulation of protein fate is a prerequisite for successful bone tissue repair. As a ubiquitin‐specific protease (USP), USP26 can stabilize the protein fate of β‐catenin to promote the osteogenic activity of mesenchymal cells (BMSCs) and significantly increased bone regeneration in bone defects in aged mice. However, direct transfection of Usp26 in vivo is inefficient. Therefore, improving the efficient expression of USP26 in target cells is the key to promoting bone tissue repair. Herein, 3D printing combined with microfluidic technology is applied to construct a functional microunit (protein fate regulating functional microunit, denoted as PFFM), which includes GelMA microspheres loaded with BMSCs overexpressing Usp26 and seeded into PCL 3D printing scaffolds. The PFFM provides a microenvironment for BMSCs, significantly promotes adhesion, and ensures cell activity and Usp26 supplementation that stabilizes β‐catenin protein significantly facilitates BMSCs to express osteogenic phenotypes. In vivo experiments have shown that PFFM effectively accelerates intervertebral bone fusion. Therefore, PFFM can provide new ideas and alternatives for using USP26 for intervertebral fusion and other hard‐to‐repair bone defect diseases and is expected to provide clinical translational potential in future treatments. Ubiquitin‐specific protease 26(USP26) can stabilize the protein fate of β‐catenin to promote the osteogenic activity of BMSCs and significantly increase bone regeneration. The protein fate regulating functional microunit (PFFM) engineered via microfluidic and 3D printing technology could provide a microenvironment for BMSCs, significantly promote adhesion, and ensure cell activity. Usp26 supplementation stabilizing β‐catenin protein significantly facilitates BMSCs to accelerate intervertebral bone fusion.