Details

Autor(en) / Beteiligte

• Nashimoto, Yuji
• Konno, An
• Imaizumi, Takuto
• Nishikawa, Kaori
• Ino, Kosuke
• Hori, Takeshi
• Kaji, Hirokazu
• Shintaku, Hirofumi
• Goto, Masafumi
• Shiku, Hitoshi

Titel

Microfluidic vascular formation model for assessing angiogenic capacities of single islets

Ist Teil von

• Biotechnology and bioengineering, 2024-03, Vol.121 (3), p.1050-1059

Ort / Verlag

United States: Wiley Subscription Services, Inc

Erscheinungsjahr

2024

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Pancreatic islet transplantation presents a promising therapy for individuals suffering from type 1 diabetes. To maintain the function of transplanted islets in vivo, it is imperative to induce angiogenesis. However, the mechanisms underlying angiogenesis triggered by islets remain unclear. In this study, we introduced a microphysiological system to study the angiogenic capacity and dynamics of individual islets. The system, which features an open‐top structure, uniquely facilitates the inoculation of islets and the longitudinal observation of vascular formation in in vivo like microenvironment with islet‐endothelial cell communication. By leveraging our system, we discovered notable islet−islet heterogeneity in the angiogenic capacity. Transcriptomic analysis of the vascularized islets revealed that islets with high angiogenic capacity exhibited upregulation of genes related to insulin secretion and downregulation of genes related to angiogenesis and fibroblasts. In conclusion, our microfluidic approach is effective in characterizing the vascular formation of individual islets and holds great promise for elucidating the angiogenic mechanisms that enhance islet transplantation therapy. A microphysiological system to examine islet angiogenic capacity reveals significant heterogeneity in their angiogenic capacities between islets, partially linked to gene expression associated with angiogenesis, fibroblast migration, and insulin secretion. This innovative microfluidic approach holds tremendous potential for advancing our understanding of the mechanisms underlying enhanced islet transplantation.