Details

Autor(en) / Beteiligte

• de Souza, Mariana Quaresma
• Bierhals, Dienefer Venske
• Reis, Ana Julia
• Chimara, Erica
• Vianna, Júlia Silveira
• von Groll, Andrea
• da Silva, Pedro Eduardo Almeida
• Ramis, Ivy Bastos

Titel

2,3,5-TRIPHENYL TETRAZOLIUM CHLORIDE AS COLORIMETRIC INDICATOR FOR DRUG SUSCEPTIBILITY TESTING AGAINST NONTUBERCULOUS MYCOBACTERIA

Ist Teil von

• Diagnostic microbiology and infectious disease, 2024-01, Vol.108 (1), p.116096-116096, Article 116096

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• •TTC is a promising tetrazolium salt to be used as a colorimetric indicator;•TTC assay could identify antimicrobial resistance in nontuberculous mycobacteria;•TTC assay allows an easier reading of results. This study aimed to propose and evaluate a drug susceptibility testing (DST) using the 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator against Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii strains, main nontuberculous mycobacteria (NTM) of clinical relevance. Our results demonstrated that the assay using TTC and the broth microdilution method recommended by the Clinical & Laboratory Standards Institute had essential agreement above 91%, 92%, and 100%, for drugs tested against MABC, MAC and M. kansasii strains, respectively. Categorical agreement above 91% was obtained for most drugs tested against MABC, except to cefoxitin (76.5%). For drugs tested against MAC and M. kansasii, categorical agreement above 92% and 100% was observed, respectively. TTC showed to be a promising colorimetric indicator of growth to be used in DST for NTM, allowing an easier reading of results.