Details

Autor(en) / Beteiligte

• Somoskői, Bence
• Bordás, Lilla
• Uno, Fusa
• Kispál, Dóra
• Müller, Linda
• Török, Dóra
• Cseh, Sándor

Titel

Effects of different cryopreservation methods on canine isolated preantral follicles

Ist Teil von

• Animal reproduction science, 2023-11, Vol.258, p.107361-107361, Article 107361

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2023

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The aim of the present study was to compare the survival and developmental rate of canine isolated preantral follicles (PAFs) after cryopreservation with different methods (closed vs open vitrification). Follicles were isolated from ovaries randomly divided into three groups: fresh control, OPS (open pulled straw) vitrified and cryotube (CT) vitrified. Post-thaw viability of follicles and oocytes was assessed. Fresh and vitrified/thawed PAFs were cultured in 20 µl drops of FSH-supplemented medium for 10 days. Follicular growth, survival rate, estradiol production and ovulation rate were examined. CT method resulted in lower rate of live cells (58.7%) and oocytes (38.8%) than that of fresh ones (83.6% and 64%, respectively) and OPS (80.3% and 79.3%, respectively). Survival rate was similar to fresh follicles in OPS group (98.5% and 95.4%, respectively), while CT decreased the survival to 81.2%. Fresh follicles showed continuous growth, while CT follicles stopped to increase their size after 2 day. In the OPS vitrified follicles, this halting occurred between Day5 and Day10. Fresh follicles showed the highest estradiol production (range: 26.9 – 266.2 pg/ml). Comparing the two vitrified groups, lower estradiol concentration range was measured in the CT group (7.8–48.7 pg/ml vs. 15.4–89.6 pg/ml). Ovulation rate in each group was lowest in the OPS group (1.7% vs 7% and 8.9% in fesh and CT, respectively). Our data show that OPS vitrification provides superior survival rate, in vitro growth and hormonal production to CT. To our knowledge, these are the first results on comparing different cryopreservation protocols on canine isolated preantral follicles. •Canine preantral follicles were cryopreserved with open and closed vitrification.•Post-thaw survival of follicles and oocytes inside was better in open system.•In vitro growth rate and estradiol production was affected by the vitrification.•OPS follicles showed intensive growth and higher hormone production than that of CT.•OPS is a suitable method to preserve canine preantral follicles.