Details

Autor(en) / Beteiligte

• Jo, Sungsin
• Lee, Seung Hoon
• Jeon, Chanhyeok
• Jo, Hye-Ryeong
• You, Yong Jin
• Lee, Jin Kyu
• Sung, Il-Hoon
• Kim, Tae-Hwan
• Lee, Chang-Hun

Titel

Myosin heavy chain 2 (MYH2) expression in hypertrophic chondrocytes of soft callus provokes endochondral bone formation in fracture

Ist Teil von

• Life sciences (1973), 2023-12, Vol.334, p.122204-122204, Article 122204

Ort / Verlag

Elsevier Inc

Erscheinungsjahr

2023

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Muscle–bone interactions during fracture healing are rarely known. Here we investigated the presence and significance of myosin heavy chain 2 (MYH2), a component of myosin derived from muscles, in fracture healing. We collected five hematoma and seven soft callus tissues from patients with distal radius fractures patients, randomly selected three of them, and performed a liquid chromatography–mass spectrometry (LC-MS) proteomics analysis. Proteomic results were validated by histological observation, immunohistochemistry, and immunofluorescence for MYH2 expression. These findings were further confirmed in a murine femoral fracture model in vivo and investigated using various methods in vitro. The LC-MS proteomics analysis showed that MYH proteins were enriched in human soft calluses compared to hematoma. Notably, MYH2 protein is upregulated as high rank in each soft callus. The histological examination showed that MYH2 expression was elevated in hypertrophic chondrocytes within the human soft callus. Consistent with human data, Myh2 were significantly co-localized with Sox9 in hypertrophic chondrocytes of murine femoral fracture, in comparison to pre-hypertrophic and proliferating chondrocytes. Soluble MYH2 protein treatment increased MMP13 and RUNX2 expression in chondrocytes. In soluble MYH2 treatment, proliferation of chondrocytes was not altered, but the osteogenic and chondrogenic features of chondrocytes increased and decreased during differentiation, respectively. These findings indicate the potential of soluble MYH2 protein as a promising therapeutic strategy for promoting endochondral bone formation in chondrocytes following fracture. •MYH2 is substantially higher in soft callus than in hematoma.•MYH2 and SOX9 are more significantly co-localized in hypertrophic chondrocytes of soft callus.•MYH2 treatment leads to an increase in the osteogenic differentiation of chondrocytes and decreases in chondrogenic differentiation.