Details

Autor(en) / Beteiligte

• Edelkamp, Janin
• Lousada, Marta B.
• Pinto, Daniela
• Chéret, Jérémy
• Calabrese, Francesco Maria
• Jiménez, Francisco
• Erdmann, Hanieh
• Wessel, Julia
• Phillip, Bodo
• Angelis, Maria De
• Rinaldi, Fabio
• Bertolini, Marta
• Paus, Ralf

Titel

Management of the human hair follicle microbiome by a synthetic odorant

Ist Teil von

• Journal of dermatological science, 2023-11, Vol.112 (2), p.99-108

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2023

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Human scalp hair follicles (HFs) engage in olfactory receptor (OR)-dependent chemosensation. Activation of olfactory receptor family 2 subfamily AT member 4 (OR2AT4) by the synthetic, sandalwood-like odorant Sandalore® up-regulated HF antimicrobial peptide expression of dermcidin (DCD), thought to be produced exclusively by sweat and sebaceous glands. To understand if intrafollicular DCD production can be stimulated by a commonly used cosmetic odorant, altering human HF microbiome composition in a clinically beneficial manner. DCD expression was compared between fresh-frozen scalp biopsies and microdissected, full-length scalp HFs, organ-cultured in the presence/absence of the OR2AT4 agonist, Sandalore® and/or antibiotics and/or the competitive OR2AT4 antagonist, Phenirat®. Amplicon-based sequencing and microbial growth assays were performed to assess how this treatment affected the HF microbiome. Synthetic odorant treatment upregulated epithelial DCD expression and exerted antimicrobial activity in human HFs ex vivo. Combined antibiotic and odorant treatment, during an ex vivo dysbiosis event, prevented HF tissue damage and favoured a more physiological microbiome composition. Sandalore®-conditioned medium, containing higher DCD content, favoured Staphylococcus epidermidis and Malassezia restricta over S. aureus and M. globosa, while exhibiting antimicrobial activity against Cutibacterium acnes. Effects reversed by co-administration of Phenirat®. We provide the first proof-of-principle that a cosmetic odorant impacts the human HF microbiome by up-regulating antimicrobial peptide production in an olfactory receptor-dependent manner. Specifically, a sandalwood-like odorant stimulates intrafollicular DCD production, likely via OR2AT4, and thereby controls microbial overgrowth. Thus, deserving further exploration as an adjuvant therapeutic in the management of folliculitis and dysbiosis-associated hair diseases. •A synthetic odorant upregulates intrafollicular antimicrobial peptide expression•Under dysbiotic conditions, odorant/antibiotic co-treatment prevents hair follicle damage•Antibiotic and synthetic odorant treatment favors a more physiological microbiome•Odorant-induced dermcidin production regulates Staphylococcus and Malassezia•Odorant effects on follicular microbiome are olfactory receptor 2AT4-mediated