Journal of cellular physiology, 2023-12, Vol.238 (12), p.2867-2878
2023
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- Autor(en) / Beteiligte
- Titel
- Regulatory effect of NK homeobox 1 (NKX2.1) on melanocortin 4 receptor (Mc4r) promoter in Mandarin fish
- Ist Teil von
- Journal of cellular physiology, 2023-12, Vol.238 (12), p.2867-2878
- Ort / Verlag
- United States: Wiley Subscription Services, Inc
- Erscheinungsjahr
- 2023
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- The melanocortin 4 receptor (MC4R) is a G protein‐coupled transporter that mediates the regulation of thyroid hormones and leptin on energy balance and food intake. However, the mechanisms of transcriptional regulation of Mc4r by thyroid hormone and leptin in fish have been rarely reported. The messenger RNA expression of Mc4r gene was significantly higher in brain than those in other tissues of mandarin fish. We analyzed the structure and function of a 2029 bp sequence of Mc4r promoter. Meanwhile, overexpression of NKX2.1 and incubation with leptin significantly increased Mc4r promoter activity, but triiodothyronine showed the opposite effect. In addition, mutations in the NKX2.1 binding site abolished not only the activation of Mc4r promoter activity by leptin but also the inhibitory effect of thyroid hormones on Mc4r promoter activity. In summary, these results suggested that thyroid hormones and leptin might regulate the transcriptional expression of Mc4r through NKX2.1.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-9541eISSN: 1097-4652DOI: 10.1002/jcp.31139Titel-ID: cdi_proquest_miscellaneous_2878711196
- Format
- –
- Schlagworte
- Animal tissues, Animals, appetite regulation, Binding sites, DNA-Binding Proteins - genetics, Energy balance, Fish, Fish Proteins - genetics, Fish Proteins - metabolism, Fishes - genetics, Fishes - metabolism, Food intake, Gene expression, Gene regulation, Genes, Homeobox, HEK293 Cells, Homeobox, Hormones, Humans, Leptin, Leptin - genetics, Leptin - pharmacology, Mandarin fish, Melanocortin, Melanocortin MC4 receptors, melanocortin‐4 receptor, Promoter Regions, Genetic - genetics, Protein transport, Receptor, Melanocortin, Type 4 - genetics, Receptor, Melanocortin, Type 4 - metabolism, Receptors, Structure-function relationships, Thyroid, Thyroid gland, Thyroid Hormones, Thyroid Nuclear Factor 1 - genetics, Thyroid Nuclear Factor 1 - metabolism, Thyroid transcription factor 1, transcriptional regulation, Triiodothyronine