Details

Autor(en) / Beteiligte

• Zhang, Yangling
• Song, Yuxin
• Zhang, Jiao
• Li, Lanlan
• He, Lin
• Bo, Jiahui
• Gong, Zhihua
• Xiao, Wenjun

Titel

L-theanine regulates the immune function of SD rats fed high-protein diets through the FABP5/IL-6/STAT3/PPARα pathway

Ist Teil von

• Food and chemical toxicology, 2023-11, Vol.181, p.114095-114095, Article 114095

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

2023

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The protein levels in a diet are correlated with immunity but the long-term intake of excessive protein can compromise various aspects of health. L-theanine regulates immunity and protein metabolism; however, how its regulatory immunity effects under a high-protein diet are unclear. We used proteomics, metabonomics, and western blotting to analyze the effects of diets with different protein levels on immune function in rats to determine the role of L-theanine in immunity under a high-protein diet. The long-term intake of high-protein diets (≥40% protein) promoted oxidative imbalance and inflammation. These were alleviated by L-theanine. High-protein diets inhibited peroxisome proliferator-activated receptor (PPAR)α expression through the interleukin (IL)-6/signal transducer and activator of transcription (STAT)3 pathway and mediated inflammation. L-theanine downregulated anti-fatty acid-binding protein 5 (FABP5), inhibited the IL-6/STAT3 axis, and reduced high-protein diet-induced PPARα inhibition. Therefore, L-theanine alleviates the adverse effects of high-protein diets via the FABP5/IL-6/STAT3/PPARα pathway and regulates the immunity of normally fed rats through the epoxide hydrolase (EPHX)2/nuclear factor-kappa B inhibitor (IκB)α/triggering receptor expressed on myeloid cells (TREM)1 axis. •Long-term intake of high protein diets of 40% protein or higher will cause oxidative imbalance, inflammatory reactions, and liver damage, which is not conducive to immunity.•LTA can inhibit the oxidative imbalance and inflammatory reaction caused by high protein diet, and alleviate liver injury.•LTA can inhibit the inflammatory response through the FABP5-IL6-STAT3-PPARα pathway.•LTA can reduce the harmful effects of high protein diet on immunity by regulating the production of inflammatory factors.