Details

Autor(en) / Beteiligte

• Jing, Yaobin
• Jiang, Xiaoyu
• Ji, Qianzhao
• Wu, Zeming
• Wang, Wei
• Liu, Zunpeng
• Guillen-Garcia, Pedro
• Esteban, Concepcion Rodriguez
• Reddy, Pradeep
• Horvath, Steve
• Li, Jingyi
• Geng, Lingling
• Hu, Qinchao
• Wang, Si
• Belmonte, Juan Carlos Izpisua
• Ren, Jie
• Zhang, Weiqi
• Qu, Jing
• Liu, Guang-Hui

Titel

Genome-wide CRISPR activation screening in senescent cells reveals SOX5 as a driver and therapeutic target of rejuvenation

Ist Teil von

• Cell stem cell, 2023-11, Vol.30 (11), p.1452-1471.e10

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2023

Quelle

ScienceDirect

Beschreibungen/Notizen

• Our understanding of the molecular basis for cellular senescence remains incomplete, limiting the development of strategies to ameliorate age-related pathologies by preventing stem cell senescence. Here, we performed a genome-wide CRISPR activation (CRISPRa) screening using a human mesenchymal precursor cell (hMPC) model of the progeroid syndrome. We evaluated targets whose activation antagonizes cellular senescence, among which SOX5 outperformed as a top hit. Through decoding the epigenomic landscapes remodeled by overexpressing SOX5, we uncovered its role in resetting the transcription network for geroprotective genes, including HMGB2. Mechanistically, SOX5 binding elevated the enhancer activity of HMGB2 with increased levels of H3K27ac and H3K4me1, raising HMGB2 expression so as to promote rejuvenation. Furthermore, gene therapy with lentiviruses carrying SOX5 or HMGB2 rejuvenated cartilage and alleviated osteoarthritis in aged mice. Our study generated a comprehensive list of rejuvenators, pinpointing SOX5 as a potent driver for rejuvenation both in vitro and in vivo. [Display omitted] •CRISPRa screening identifies a comprehensive set of rejuvenators against senescence•Activation of SOX5 elicits the rejuvenation program through epigenetic remodeling•SOX5 activates the HMGB2 enhancer for subsequent geroprotective effects•Gene therapy utilizing SOX5 alone can promote the regeneration of aged knee joints Jing et al. performed genome-wide CRISPRa screening, pinpointing SOX5 as a potent rejuvenator of senescent human cells. Through epigenetic and transcriptional remodeling, SOX5 activates HMGB2, imparting geroprotective effects. Gene therapy with SOX5 or HMGB2 alone can mitigate osteoarthritis in aged mice, achieving comparable rejuvenation as the combination of Yamanaka factors.