Details

Autor(en) / Beteiligte

• Yao, Chixuan
• Hu, Yaozhong
• Liu, Qisijing
• Liu, Jing-Min
• Ji, Xuemeng
• Lv, Huan
• Wang, Shuo

Titel

Nanobody mediated dual-mode immunoassay for detection of peanut allergen Ara h 3

Ist Teil von

• Food chemistry, 2024-03, Vol.436, p.137679-137679, Article 137679

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

2024

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •Nanobody-based dual-mode immunoassay for Ara h 3 was developed with LOD of 6.61 ng/mL.•The dominance of nanobody and B/N-CDs improved the accuracy of Ara h 3 immunoassay.•The method achieved the assessment of Ara h 3 in peanut contamination foodstuffs. To improve the performance of peanut allergen Ara h 3 detection, depending on boron and nitrogen carbon dots (B/N-CDs), a nanobody (Nb) mediated dual-mode immunoassay was established, which combines the dominance of colorimetry with ratiometric fluorescence techniques. With the catalysis of Horseradish peroxidase (HRP), the oxidization of o-phenylenediamine (o-PD) in the presence of H2O2, leading to the production of yellow 2,3-diaminophenolazine (DAP) with an absorption peak at 431 nm. Owing to inner filter effect (IFE), DAP quenched the fluorescence of B/N-CDs at 426 nm, and it emerged a new emission peak at 549 nm. The fluorescence intensity ratio and absorption intensity can be utilized for quantitative analysis of Ara h 3 concentration. Under optimal conditions, the detection limits were 6.61 and 9.79 ng·mL−1, respectively. The dual-mode immunoassay was assessed containing specificity, stability, reproducibility, and practicability. This method paved the way for sensitive detection of Ara h 3 without background Interference.