Details

Autor(en) / Beteiligte

• Mustafa, Ghulam
• Zahid, Muhammad Tariq
• Bharat Kurade, Mayur
• Mahadeo Patil, Swapnil
• Shakoori, Farah Rauf
• Shafiq, Zeeshan
• Ihsan, Sidra
• Ahn, Yongtae
• Khan, Azmat Ali
• Gacem, Amel
• Jeon, Byong-hun

Titel

Molecular characterization of azoreductase and its potential for the decolorization of Remazol Red R and Acid Blue 29

Ist Teil von

• Environmental pollution (1987), 2023-10, Vol.335, p.122253, Article 122253

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2023

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Azoreductase is a reductive enzyme that efficiently biotransformed textile azo dyes. This study demonstrated the heterologous overexpression of the azoreductase gene in Escherichia coli for the effective degradation of Remazol Red-R and Acid-Blue 29 dyes. The AzK gene of Klebsiella pneumoniae encoding a ≈22 kDa azoreductase enzyme was cloned into the pET21+C expression vector. The inoculum size of 1.5%, IPTG concentration of 0.5 mM, and incubation time of 6 h were optimized by response surface methodology a statistical tool. The crude extract showed 76% and 74%, while the purified enzyme achieved 94% and 93% decolorization of RRR and AB-29, respectively in 0.3 h. The reaction kinetics showed that RRR had a Km and Vmax value of 0.058 mM and 1416 U mg−1, respectively at an NADH concentration of 10 mM. HPLC and GC-MS analyses showed that RRR was effectively bio-transformed by azoreductase to 2-[3-(hydroxy-amino) benzene-1-sulfonyl and AB-29 to aniline and 3-nitrosoaniline. This study explored the potential of recombinant azoreductase isolated from K. pneumoniae in the degradation of toxic textile azo dyes into less toxic metabolites. [Display omitted] •Azoreductase gene overexpression in E. coli for effective degradation of azo dyes.•Optimization of protein expression and decolorization using RSM.•Purified enzyme decolorized 94 and 93% of RRR and AB-29, respectively in 0.3 h.•HPLC and GCMS confirmed effective degradation of azo dyes into simple compounds.